烟草科技2017，Vol.50Issue(6)：1-8,8.
烟草酪氨酸转氨酶基因家族分析
Analysis of tyrosine aminotransferase gene family in tobacco
摘要
Abstract
To reveal the characteristics and functions of tyrosine aminotransferase (TAT) gene family in tobacco, bioinformatics was used to identify and analyze TAT gene families from different tobacco varieties. The results showed that 4, 3 and 3 TAT genes were identified in the genomes of Nicotiana tabacum, Nicotiana sylvestris, and Nicotiana tomentosiformis, respectively. In subfamily Ⅰ, TATs were 1263-1338 bp in length, encoded 404-445 amino acids with molecular weights in 42.81-49.64 kD and the predicted TAT proteins were stable proteins with no transmembrane domains. In subfamily Ⅱ, the predicted TAT proteins were instable proteins. Except for NtabTAT4, the other TATs consisted of 7 exons and 6 introns. Phylogenetic analysis indicated that tobacco TAT proteins could be clustered into two subgroups: Group Ⅰ and Group Ⅱ. qPCR showed that NtabTATs were expressed in roots, stalks, leaves and flowers.关键词
烟草/酪氨酸转氨酶/基因家族/进化分析Key words
Tobacco/Tyrosine aminotransferase (TAT)/Gene family/Phylogenetic analysis分类
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赵影影,祁超亚,位芳,王燃,王姗姗,张剑锋,金立锋,陈霞,魏攀,王中,郑庆霞,许亚龙..烟草酪氨酸转氨酶基因家族分析[J].烟草科技,2017,50(6):1-8,8.基金项目
中国烟草总公司烟草基因组计划重大专项项目"基于基因组编辑技术的烟草重要基因功能研究"[110201501015 (JY-02) ]. （JY-02）
