烟草科技2017，Vol.50Issue(6)：16-20,32,6.
温度和品种抗性对烟草青枯病潜育期的影响
Effects of temperature and varietal resistance on latent period of tobacco bacterial wilt
摘要
Abstract
To explore the occurrence pattern of tobacco bacterial wilt (TBW), spread plate method was used to determine the concentration of bacteria suspension. The pot experiments on cv. K326, Honghuadajinyuan, Yunyan 85 and Yunyan 87 were carried out to screen out the optimal artificial inoculation method. The key factors influencing the infection threshold and latent period of TBW were determined using this method. The results showed that root-dipping was the optimal artificial inoculation method for Ralstonia solanacearum. The occurrence time of TBW with root-dipping inoculation was 8 days earlier than that with root-irrigation inoculation. Through the inoculation tests of different concentrations, the infection threshold of R. solanacearum was determined as 3×102 cfu/mL. Further, the results of the pot experiments in the artificial climatic incubator indicated that four tobacco varieties had a similar pattern: the latent period of TBW was significantly affected by temperature, latent period decreased with the rising of temperature. In the optimal occurrence time, the latent period of TBW was between 20-28 days, with 24 days on average. The latent period in varieties with a higherdisease resistance was longer.关键词
烟草青枯病菌/潜育期/侵染阈值/温度/品种Key words
Ralstonia solanacearum/Latent period/Infection threshold/Temperature/Variety分类
农业科技引用本文复制引用
何永宏,曾乙心,刘林,赵明丽,刘月静,陈兴全,谢勇,秦西云..温度和品种抗性对烟草青枯病潜育期的影响[J].烟草科技,2017,50(6):16-20,32,6.基金项目
中国烟草总公司云南省公司资助项目"烟草主要土传病害快速预警技术研究及应用"(2015YN07) （2015YN07）
云南省高校科技创新团队支持计划项目"云南高校经济作物重要病原物致病机理与防控科技创新团队"〔云教科(2014)22 号〕. （2014）
