烟草科技2017，Vol.50Issue(6)：21-25,5.
肿柄菊中倍半萜类化合物对番茄斑萎病毒的抑制活性分析
Inhibition effects of sesquiterpenoids from Tithonia diversifolia on tomato spotted wilt virus
摘要
Abstract
To effectively control plant viral diseases caused by tomato spotted wilt virus (TSWV), antiviral lead compounds were extracted from plants. Lesion counting was used to detect the activities of TSWV after treating with 14 sesquiterpenoids from Tithonia diversifolia. The results showed that applying 14 sesquiterpenoids (100 μg/mL) after inoculation with virus for 24 h, the inhibition rates of seven sesquiterpenoids were higher than Ningnanmycin (CK, 58.80%) in control experiment. The inhibition rate of sesquiterpenoids-12 was the highest (76.00%). Inoculated with virus after applying 14 sesquiterpenoids for 6 h, the inhibition rates of four sesquiterpenoids were higher than Ningnanmycin (61.25%), and the inhibition rate of sesquiterpenoids-12 was 78.50%. Inhibition rate of sesquiterpenoids increased with the rising of concentration, which showed a concentration-dependent manner. In control and protection experiments, when the concentration was 200 μg/mL, the inhibition rates of sesquiterpenoids-12 were 86.00% and 89.20%, respectively. Therefore, sesquiterpenoids from Tithonia diversifolia could be used as lead compounds of biopesticides in resistance to TSWV.关键词
烟草/番茄斑萎病毒(TSWV)/肿柄菊/倍半萜/抑制率Key words
Tobacco/Tomato spotted wilt virus (TSWV)/Tithonia diversifolia/Sesquiterpenoids/Inhibition rate分类
农业科技引用本文复制引用
赵立华,董家红,苏晓霞,李顺林,张洁,徐弢,周晓吉,张仲凯,陈海如..肿柄菊中倍半萜类化合物对番茄斑萎病毒的抑制活性分析[J].烟草科技,2017,50(6):21-25,5.基金项目
云南省高端科技人才引进项目"云南茄果类蔬菜病毒病的监测预警与绿色防控技术体系的研究与应用"(2013HA028) （2013HA028）
云南省科技计划项目"主要农作物病毒病监测防控技术研究与应用"(2012CH007) （2012CH007）
云南省中青年学术技术带头人后备人才项目(2015HB081). （2015HB081）
