眼科新进展2017，Vol.37Issue(7)：615-618,4.DOI:10.13389/j.cnki.rao.2017.0156
局部应用腺苷A2a受体拮抗剂对慢性高眼压大鼠视网膜谷氨酰胺合成酶(GS)和L-谷氨酸-L-天冬氨酸转运体(GLAST)表达的影响
Effects of A2a adenosine receptor antagonist SCH442416 and ZM241385 on GS and GLAST expression in rat retina under chronic intraocular hypertension
摘要
Abstract
Objective To observe the effects of A2a adenosine receptor antagonist SCH442416 and ZM241385 on the expression of glutamine synthetase(GS) and L-Glutamate/L-Aspartate Transporter(GLAST) in rat retina under chronic ocular hypertension model.Methods Rat chronic ocular hypertension models were induced in the right eye of 12 male Sprague Dawley rats by blocking three episcleral veins,the left eye as control one.Intraocular pressure (IOP) was measured and compared at postoperative 1 week,2 weeks and 3 weeks.54 male chronic ocular hypertension rats were divided into 3 groups randomly,topically applying A2a adenosine receptor antagonist SCH442416,ZM241385 and carrier,respectively,three times a day for three weeks.At three weeks,mRNA and protein expression of GS and GLAST in rat retina were analyzed by RealTime-PCR and Western-blot.Results The average IOP of the modeling eyes at postoperative 1 week,2 weeks and 3 weeks were higher than that of the control eyes (all P ＜ 0.05).The mRNA and protein expression of GS and GLAST in the retina of SCH442416 and ZM241385 groups increased significantly compared to the carrier group (all P ＜ 0.05).However,the differences of mRNA and protein expression of GS and GLAST between SCH442416 and ZM241385 groups was not significant(all P ＞ 0.05).Conclusion Rat chronic ocular hypertension model can be induced by blocking three episceral veins successfully and effectively.A2a adenosine receptor antagonist SCH442416 and ZM241385 increase the expression of GS and GLAST.There seems no difference between the effects of these two drugs.关键词
腺苷/高眼压/谷氨酰胺合成酶/L-谷氨酸-L-天冬氨酸转运体/鼠Key words
adenosine/intraocular hypertension/glutamamin synthetase/L-glutamate-L-aspartate transporter/rat分类
医药卫生引用本文复制引用
李勇,闵颖君,郎莉莉,钟一声,张润琦..局部应用腺苷A2a受体拮抗剂对慢性高眼压大鼠视网膜谷氨酰胺合成酶(GS)和L-谷氨酸-L-天冬氨酸转运体(GLAST)表达的影响[J].眼科新进展,2017,37(7):615-618,4.基金项目
上海市长宁区科学技术委员会资助项目(编号:CNKW2013J10).Supported by Shanghai Changning Scientific and Technological Committee (No:CNKW2013J10) （编号:CNKW2013J10）
