医疗卫生装备2017，Vol.38Issue(6)：22-26,5.DOI:10.7687/j.issn1003-8868.2017.06.022
氨基化荧光碳点制备及其荧光探针构建
Preparation and evaluation of fluorescent carbon dots (CDs) and CDs based immunofluorescence probe
摘要
Abstract
Objective To fabricate an immunofluorescence probe system of carbon dots conjugated antibody based on antigen-antibody reaction principles.Methods A green one-step microwave assisted pyrolysis method was applied to preparing fluorescent carbon dots (CDs) using aminoglucose as carbon source and the obtained CDs were conjugated with antibody via EDC/NHS reactions to build CDs based fluorescent probe.Furthermore,the properties of CDs and CDs based probe system were evaluated by Fourier transform infrared (FTIR) spectra,transmission electron microscopy (TEM),UV-vis absorption and so on.Results The as-prepared CDs showed excellent fluorescence and hydrophilicity and CDs based immunofluorescence probe exhibited the capability of quick detection of E.coli O157:H7.Cinclusion Fluorescent CDs as one new emerging environment-friendly nanomaterial has great potential in biosensors.关键词
碳点/荧光/免疫荧光探针/纳米材料/大肠杆菌Key words
carbon dots/fluorescence/immunofluorescence probe/nanomaterial/E.coli O157:H7分类
医药卫生引用本文复制引用
张春林,张勇,车望远,李钒,房玉海,洪志飞,刘长军..氨基化荧光碳点制备及其荧光探针构建[J].医疗卫生装备,2017,38(6):22-26,5.基金项目
国家自然科学基金青年项目(51303210,51502345) （51303210,51502345）
天津市应用基础与前沿技术研究计划一般项目(14JCYBJC29500) （14JCYBJC29500）
