云南农业大学学报（自然科学）2017，Vol.32Issue(3)：410-420,11.DOI:10.16211/j.issn.1004-390X(n).2017.03.004
烟草丛顶病毒云南不同分离物全基因组序列测定与分析
Determination and Analysis of the Complete Nucleotide Sequences of Different Isolates of Tobacco bushy top virus in Yunnan, China
摘要
Abstract
[Purpose] The aim of this study was to explore the genetic differences among different isolates of Tobacco bushy top virus (TBTV) in Yunnan and complete the phylogenetic analysis.[Method] The complete genomes of ten TBTV Yunnan isolates were amplified by RT-PCR.[Result] All of the genomic sequences of the ten TBTV isolates were 4 152 nucleotides (nt) in size,and contained four open reading frames (ORFs).ORF2 expressed the fusion protein through-1 frameshift with ORF1,and the sequence GGAUUUU was suggested to be the signature for the frameshift strategies of ORF1-ORF2 in TBTV.Sequence analysis results revealed the nt identity of the complete sequence of the ten TBTV isolates was ranged from 84.9％ to 99.1％,and isolate TBTV-DHo was the most variant compared with the other TBTV isolates.Recombinant events were detected among the TBTV Yunnan isolates.Phylogenetic relationships of these isolates involved in the recombinant,and recombinant was mainly occurred in the variant regions of ORF1-ORF2.ORF1 and ORF1-ORF2 were divergent among the four TBTV ORFs,while ORF4 and 3'UTR were conserved.Phylogenetic analysis showed that TBTV was most closely related to Opium poppy mosaic virus (OPMV) among different umbraviruses with nt identity of 61.1％ to 61.6％,while was more distant to Carrot mottle virus (CMoV) and Carrot mottle mimic virus (CMoMV) with nt identity of 54％-55.1％ in genus Umbravirus.Among the members in the 13 genera of family Tombusviridae,TBTV shared 25.1％-49.8％ nt sequence identity with other viruses except umbraviruses,while umbraviruses were distant with the RNA2 of Dianthoviruses in family Tombusviridae.[Conclusion] Sequence analysis indicated a high genetic diversity among the TBTV Yunnan isolates,and recombinant events occurred among these isolates.TBTV was most closely related to OPMV among different umbraviruses.关键词
烟草丛顶病毒/幽影病毒属/番茄丛矮病毒科/序列分析/重组Key words
tobacco bushy top virus/Umbravirus/tombusviridae/sequence analysis/recombinant分类
农业科技引用本文复制引用
吕婧,李凡,刘芳,谭冠林,魏薇,尹朝先,马龑,陈小姣,和理淮,陈海如..烟草丛顶病毒云南不同分离物全基因组序列测定与分析[J].云南农业大学学报（自然科学）,2017,32(3):410-420,11.基金项目
云南省高校科技创新团队支持计划(云教科[2014] 22号) （云教科[2014] 22号）
国家自然科学基金(30660101,31571983) （30660101,31571983）
云南农业大学杰出人才专项资助计划(2015JY01). （2015JY01）
