云南农业大学学报（自然科学）2017，Vol.32Issue(3)：442-449,8.DOI:10.16211/j.issn.1004-390X(n).2017.03.008
版纳微型猪近交系细胞色素P450家族基因CYP1A1克隆及生物信息学分析
Cloning and Bioinformatics Analysis of CYP1A1 of Cytochrome P450 Family from Banna Mini-pig Inbred Line
摘要
Abstract
[Purpose] Our purpose was to clone sequence and analyze the character of the cytochrome P450 family 1 subfamily A member 1 gene (CYP1A1) from Banna mini-pig inbred line (BMI).[Methods] The complete CDS of CYP1A1 gene was cloned using RT-PCR method from BMI.Nucleotide and protein sequences of CYP1A1 were utilized to carry out bioinformatics analysis and construct the phylogenetic tree.[Results] The CDS length of BMI CYP1A1 is 1 551 bp in length which encodes a protein of 516 amino acids with molecular weight (Mw) 58.39 ku,and isoelectric point (pI) 7.83,and locates in the endoplasmic reticulum with 94.1％ reliability.CYP1A1 protein contains a cytochrome P450 cysteine heme-iron ligand signature,an N-myristoylation site,a aidation site,two N-glycosylation sites,six protease C phosphorylation sites and five protein kinase Ⅱ phosphorylation sites.Phylogenetic analysis of 11 species showed that CYP1A1 of BMI (pig) had the closest relationship with these of cattle and goat.[Conclusion] The results will lay a foundation for further study on CYP1A1 gene function in BMI.关键词
细胞色素P450家族/CYP1A1基因/编码区序列/版纳微型猪近交系/生物信息学/药物代谢Key words
cytochrome P450 family/CYP1A1 gene/CDS/banna mini-pig inbred line (BMI)/bioinformatics/drug metabolism分类
农业科技引用本文复制引用
王淑燕,王配,霍金龙,查星琴,潘伟荣,张霞,王雪飞..版纳微型猪近交系细胞色素P450家族基因CYP1A1克隆及生物信息学分析[J].云南农业大学学报（自然科学）,2017,32(3):442-449,8.基金项目
国家自然科学基金项目(31660637,31460580,31660650). （31660637,31460580,31660650）
