烟台大学学报（自然科学与工程版）2017，Vol.30Issue(3)：191-196,213,7.DOI:10.13951/j.cnki.37-1213/n.2017.03.004
基于NCBI核酸数据库资源的产黄青霉微卫星序列的筛选
Screening of Microsatellite DNA in Penicillium chrysogenum basedon NCBI Nucleic Acid Database Resources
摘要
Abstract
Microsatellite sequences in Penicillium chrysogenum are screened using SSRHunter1.3 software.One hundred and sixty-three microsatellites are found in the 30 090 464 bp genome sequence.The numbers and abundance of the microsatellites are investigated using bioinformatic method.Among the 163 microsatellites in P.chrysogenum, there are 133 two-base repeats, 29 three-base repeats and one four-base repeats, accounting for 81.60%, 17.79% and 0.61% of the total microsatellites, respectively.Most of the two-base repeat sequences are AT repeats, followed by AG repeats, representing 44.79% and 23.92% of the total two-base repeats, respectively.We find nine classes of the three-base repeat sequences, AAG and ACT are the most frequent ones, followed by the ACC and AGC, accounting for 3.68%, 2.45% and 0.61% of the total microsatellites, respectively.Only one four-base repeat sequence, ATGT, is found, which accounts for 0.61% of the total microsatellites.Meanwhile, thirty PCR primer pairs for two-base repeats occurred over seven times and three-base repeats occurred over six times are designed using Primer Premier 5.0 software.This study provided useful information for microsatellite marker screening, and enriches the genomic database in P.chrysogenum, which is important for genetic structure analysis, molecular marker breeding and genetic diversity analysis.关键词
产黄青霉/微卫星序列/生物信息学/基因组/引物Key words
Penicillium chrysogenum/microsatellite sequenc/bioinformatics/genome/primer分类
生物科学引用本文复制引用
李瑶瑶,刘云国,刘凌霄,刘军,张亮,王敏强..基于NCBI核酸数据库资源的产黄青霉微卫星序列的筛选[J].烟台大学学报（自然科学与工程版）,2017,30(3):191-196,213,7.基金项目
国家自然科学基金资助项目(31560719) （31560719）
新疆自治区天山学者科研启动经费资助项目(45030). （45030）
