烟台大学学报（自然科学与工程版）2017，Vol.30Issue(3)：207-213,7.DOI:10.13951/j.cnki.37-1213/n.2017.03.007
麦角甾醇类化合物G10-10A体外抗胃癌细胞MKN45生长作用的研究
Inhibitory Effect of Ergosteroids G10-10A, an Ergosteroids Compound,on Human Gastric Cancer MKN45 Cells in vitro
摘要
Abstract
Ergosteroids, a gloeophyllins A, is isolated from the fruiting bodies of Gloeophyllum abietinum collected in the Tibet plateau.In this work, the anti-tumor activity of G10-10A on gastric cancer cells is evaluated in vitro, and the underlying mechanism of action is explored.G10-10A shows robust cytotoxic activity against MKN45 cells in the MTT assay, the cell growth curve assay, and the colony-formation assay.Treatment with G10-10A can induce the cell undergoing apoptosis in the flow cytometry assay and the western blot assay.At 24 h after treatment, G10-10A increases the protein level of P53, as well as P21 and PUMA, two downstream targets of P53.Interestingly, co-treatment with Small-Molecule inhibitors, Z-VAD-FMK or Pifithrin-α, attenuates the expression level of CL-PARP induced by G10-10A, and alleviates its cytotoxic activity.关键词
细胞凋亡/真菌/G10-10A/P53Key words
cell apoptosis/fungi/G10-10A/P53分类
医药卫生引用本文复制引用
解晓霞,王洪波,张静文,杨延婷,韩俊杰,刘宏伟,赵烽,傅风华..麦角甾醇类化合物G10-10A体外抗胃癌细胞MKN45生长作用的研究[J].烟台大学学报（自然科学与工程版）,2017,30(3):207-213,7.基金项目
国家自然科学基金资助项目(81673334,81202038). （81673334,81202038）
