应用生态学报2017，Vol.28Issue(5)：1515-1521,7.DOI:10.13287/j.1001-9332.201705.026
小兴安岭酸性泥炭土硝化作用的类型及其驱动因子
Type and key drivers of nitrification for an acidic peat soil in Xiaoxing'an Mountain, China
摘要
Abstract
Acidic forest peat soil in Xiaoxing'an Mountain was used to investigate the type and key drivers of nitrification by carrying out nitrification incubation after adding 10 mL · L-1 C2H2 and different amounts of (NH4)2SO4 (0,1.2,6.0 mmol N · kg-1).Results showed that strong mineralization (0.9-1.4 mg N · kg-1 · d-1) and nitrification (0.4-0.6 mg N · kg-1 · d-1) after 2 weeks of incubation were observed for both nitrogen and no nitrogen treatments,and no significant difference was observed for different (NH4) 2SO4 application treatments.For C2H2 treatment,there was relatively stronger mineralization (0.8 mg N · kg-1 · d-1),but no obvious nitrification (0 mg N · kg-1 d-1).These results indicated that the nitrification in acidic peat soil was mainly autotrophic,and inorganic nitrogen application did not affect nitrification rate significantly.Results also implied that the substrate (NH3) for nitrification was from the organic N mineralization,rather than the (NH4) 2SO4 application.Both ammonia-oxidizing bacteria (AOB) and archaea (AOA) abundances increased significantly during 0-14 d regardless of nitrogen application.However,no significant difference in AOB and AOA abundances for different (NH4)2SO4 application treatments was observed,indicating that ammonia oxidizers did not respond positively to (NH4)2 SO4 application.Compared with the treatments without C2H2,AOB and AOA abundances did not change significantly during the incubation in C2H2 treatment,suggesting that both AOA and AOB were likely the key players in nitrification in the acidic peat soil.关键词
酸性森林土/氨氧化古菌(AOA)/氨氧化细菌(AOB)/氮循环Key words
acidic forest soil/ammonia-oxidizing archaea (AOA)/ammonia-oxidizing bacteria (AOB)/N cycling引用本文复制引用
王智慧,苏静,蒋先军..小兴安岭酸性泥炭土硝化作用的类型及其驱动因子[J].应用生态学报,2017,28(5):1515-1521,7.基金项目
本文由国家自然科学基金项目(41671232,41271267)资助 This work was supported by the National Natural Science Foundation of China (41671232,41271267). （41671232,41271267）
