应用生态学报2017，Vol.28Issue(5)：1633-1641,9.DOI:10.13287/j.1001-9332.201705.004
大豆根系应答重金属Cd胁迫的转录组分析
Transcriptome analysis of response to heavy metal Cd stress in soybean root
摘要
Abstract
Cadmium (Cd) is a common pollutant not required for life activities,which can have extremely strong toxicity to organisms and affect the growth of crops at a low concentration in soil.To investigate the molecular mechanism of soybean root responding to Cd stress,7-day old soybean seedlings were stressed by Cd (75 μmol · L-1) for 0,4,8,12 and 48 h.Comparative transcriptome analysis showed 244,1545,442 and 1401 of genes responded to the four Cd treatments,respectively,and total 2670 differential expression genes were obtained.GO analysis classified these genes into 56 functional categories and COG analysis classified them into 25 functional categories.KEGG analysis showed that many genes involved in the phenylalanine metabolism,ubiquinone and other terpenoid-quinone biosynthesis and cysteine and methionine metabolism and so on.Further we found that expression of three isoflavones 2'-hydroxylase genes,two isoflavonereductase genes and a chalcone synthase gene were evidently up-regulated in all Cd treatments.The results of RT-PCR analysis of four DEGs were consistent with those of RNA-Seq data,further confirming the reliability of RNA-Seq results.关键词
大豆/重金属镉/转录组Key words
soybean/heavy material cadmium/transcriptome引用本文复制引用
张晓娜,朴春兰,董友魁,崔敏龙..大豆根系应答重金属Cd胁迫的转录组分析[J].应用生态学报,2017,28(5):1633-1641,9.基金项目
本文由国家自然科学基金项目(31370526)资助 This work was supported by the National Natural Science Foundation of China (31370526). （31370526）
