中国病理生理杂志2017，Vol.33Issue(7)：1171-1176,6.DOI:10.3969/j.issn.1000-4718.2017.07.003
黄芩素诱导乳腺癌细胞自噬
Baicalein induces autophagy in breast cancer cells
摘要
Abstract
AIM: To investigate the autophagy of breast cancer cells induced by baicalein and to explore its mechanism.METHODS: The effects of baicalein on the viability of MCF-7 cells and 4T1 cells were investigated by MTT assay, and the dosage of the drug was determined.The expression levels of microtubule-associated protein 1 light chain 3-II (LC3-II) and LC3-I in the MCF-7 cells and 4T1 cells treated with baicalein at doses of 25, 50 and 100 μmol/L, or combined with autophagy inhibitor 3-methyladenine (3-MA) were determined by Western blot.In order to confirm the role of baicalein in autophagy, the effect of 3-MA on the apoptosis of both MCF-7 cells and 4T1 cells induced by baicalein was analyzed by flow cytometry.The protein levels of p-mTOR, mTOR, p-AKT and AKT were examined by Western blot and the role of AKT-mTOR pathway in the induction of autophagy in breast cancer induced by baicalein was determined by the combination of activators.RESULTS: Baicalein at 50 μmol/L and above doses significantly inhibited the viability of breast cancer cells in a dose-and time-dependent manner.The expression of LC3-II/LC3-I in both MCF-7 cells and 4T1 cells was significantly enhanced after the action of baicalein, and the ratio of LC3-II/LC3-I was significantly decreased after 3-MA addition.The results of flow cytometry showed that, compared with baicalein group, the combination of baicalein and 3-MA promoted the levels of necrosis and apoptosis.Moreover, the protein levels of p-mTOR and p-AKT were significantly decreased and were rescued by EGF, while their total protein levels were not changed.CONCLUSION: Baicalein induces autophagy through AKT-mTOR pathway both in MCF-7 cells and 4T1 cells.关键词
黄芩素/乳腺癌细胞/自噬/AKT-mTOR信号通路Key words
Baicalein/Breast cancer/Autophagy/ATK-mTOR signal pathway分类
医药卫生引用本文复制引用
凌云,屠珏,蔡兆伟,蔡月琴,褚燕青,陈民利..黄芩素诱导乳腺癌细胞自噬[J].中国病理生理杂志,2017,33(7):1171-1176,6.基金项目
浙江省自然科学基金(No.LQ13H280004) （No.LQ13H280004）
浙江中医药大学校级科技创新团队(No.XTD201301) （No.XTD201301）
