中国病理生理杂志2017，Vol.33Issue(7)：1219-1225,7.DOI:10.3969/j.issn.1000-4718.2017.07.011
下丘脑室旁核CRH神经元激活在慢性充血性心力衰竭中的交感兴奋作用
Activation of corticotrophin releasing hormone-containing neurons in hypothalamic paraventricular nucleus contributes to sympathoexcitation in rats with congestive heart failure
摘要
Abstract
AIM: To observe the expression of corticotropin releasing hormone (CRH) within the paraventricular nucleus of hypothalamus (PVN) and to explore the relationship between the activated CRH-containing neurons and sympathetic activity in rats with heart failure (HF).METHODS: Healthy male Sprague-Dawley (SD) rats were subjected to coronary artery ligation to induce HF, and chronic intracerebroventricular (ICV) infusion was performed by osmotic pump for 4 weeks.The rats in sham group and HF group were given vehicle (VEH;artificial cerebrospinal fluid 0.25 μL/h).The rats in HF plus treatment group were treated with CRH competitive inhibitor αh-CRH (15 mg/h).Meanwhile, the Lewis rats and Fischer 344 rats for control study also underwent coronary ligation to induce HF or sham surgery.After 4 weeks, left ventricular end-diastolic pressure (LVEDP) and maximum positive/negative change in pressure over time (±dp/dtmax) were determined.The right ventricular-to-body weight (RV/BW) and lung-to-body weight (lung/BW) ratios were calculated.The renal sympathetic nerve activity (RSNA) was recorded and the plasma norepinephrine (NE) level was measured.The expression of CRH in the PVN combined with the plasma adrenocorticotrophic hormone (ACTH) levels were measured.RESULTS: Compared with the sham-SD rats, the HF-SD rats had a greater number of CRH positive neurons in the PVN (accordingly the plasma ACTH levels were increased), accompanied by decreased ±dp/dtmax and increased RSNA, plasma NE, LVEDP, lung/BW and RV/BW.However, ICV treatment with αh-CRH attenuated these changes in the HF-SD rats (P<0.05).Compared with the sham-Fisher 344 rats, the HF-Fisher 344 rats also had a greater number of CRH positive neurons in the PVN (accordingly the plasma ACTH levels were increased).In addition, they had significantly increased RSNA and plasma NE level, higher LVEDP, RV/BW and lung/BW, and lower ±dp/dtmax (P<0.05).Compared with the SHAM-Lewis rats, the HF-Lewis rats had not significantly changed in the above parameters.CONCLUSION: In CHF, the CRH-containing neurons in PVN are activated, thus aggravating cardiac function by increasing sympathoexcitation.关键词
慢性充血性心力衰竭/下丘脑室旁核/促肾上腺皮质激素释放激素-神经元/交感神经系统/Lewis大鼠/Fisher344大鼠Key words
Chronic congestive heart failure/Paraventricular nucleus of hypothalamus/Corticotropin releasing hormone-containing neurons/Sympathetic nervous system/Lewis rat/Fisher 344 rat分类
医药卫生引用本文复制引用
郭艳琳,张华屏,杨彩红,吕豆,康玉明..下丘脑室旁核CRH神经元激活在慢性充血性心力衰竭中的交感兴奋作用[J].中国病理生理杂志,2017,33(7):1219-1225,7.基金项目
山西省自然科学基金资助项目(No.2014011043-6) （No.2014011043-6）
山西医科大学博士启动基金项目(No.03201101) （No.03201101）
山西医科大学基础医学院331基础医学科技培植基金项目(No.201412) （No.201412）
