中国病理生理杂志2017，Vol.33Issue(7)：1328-1331,4.DOI:10.3969/j.issn.1000-4718.2017.07.029
穿心莲内酯对卵巢癌细胞株SKOV-3侵袭与凋亡的影响
Effects of andrographolide on invasion and apoptosis of ovarian cancer SKOV-3 cells
摘要
Abstract
AIM: To investigate the effects of andrographolide on the invasion and apoptosis of ovarian cancer cell line SKOV-3, and to explore the possible mechanisms.METHODS: SKOV-3 cells were treated with different concentrations (0, 5, 10, 20 or 40 μmol/L) of andrographolide for different time (12, 24, 36 or 48 h), and then the cell viability was determined by CCK-8 assay.The cell invasion ability was analyzed by Transwell assay and cell apoptosis was detected by TUNEL staining.The protein levels of p-PI3K, p-Akt and p-mTOR were examined by Western blot.RESULTS: The results of CCK-8 assay revealed that andrographolide inhibited the growth of SKOV-3 cells in a dose-and time-dependent manner.Treatment with andrographolide at 20 μmol/L for 36 h significantly decreased the invasion ability of SKOV-3 cells, while increased cell apoptosis.In addition, the protein levels of p-PI3K, p-Akt and p-mTOR were reduced after andrographolide treatment.CONCLUSION: Andrographolide inhibits the growth and invasion of ovarian cancer SKOV-3 cells by suppression of PI3K/Akt/mTOR signaling pathway.关键词
穿心莲内酯/卵巢癌细胞/PI3K/Akt/mTOR信号通路Key words
Andrographolide/Ovarian cancer cells/PI3K/Akt/mTOR signaling pathway分类
医药卫生引用本文复制引用
苏芳静,张斌,田林燕..穿心莲内酯对卵巢癌细胞株SKOV-3侵袭与凋亡的影响[J].中国病理生理杂志,2017,33(7):1328-1331,4.基金项目
河南省教育厅高校重点科研项目(No.15A360011) （No.15A360011）
