中国动脉硬化杂志2017，Vol.25Issue(7)：661-665,5.
miR-626下调HepG2细胞载脂蛋白(a)的表达
Hsa-miR-626 down-regulates expression of apolipoprotein (a) in HepG2 cells
摘要
Abstract
Aim To screen miRNA for efficient regulation of LPA gene expression.Methods The miRNA of LPA gene 3'UTR were predicted by on-line prediction tools.The mRNA and protein expression of apolipoprotein (a) (Apo (a)) were detected by RT-PCR and Western blot after transfection.The experiment was divided into 4 groups:control group,miR-626 mimic group,miR-626 mimic+miR-626 inhibitor group and miR-626 inhibitor group.The miR-626 target validation assay was performed using a luciferase reporter system.Results There were 9 miRNA binding 3'UTR of LPA gene.Compared with the control group,the expression of Apo (a) mRNA was not significantly different between the mimic transfected group and the control group,but miR-626 significantly down-regulated the expression of Apo(a) protein.MiR-626 attenuated Apo(a) protein down-regulation by miR-626 inhibitor.The fluorescence intensity of the miR-626 cells was significantly lower than that of the control group after cell lysis.Conclusion MiR-626 down-regulates the expression of Apo(a) in HepG2 cells.MiR-626 down-regulates the expression of Apo(a) protein in LPA mRNA by direct binding to LPA mRNA 3'UTR.关键词
载脂蛋白(a)/HepG2细胞/miR-626Key words
Apolipoprotein (a)/HepG2 cell/MiR-626分类
医药卫生引用本文复制引用
孟军,杨简,何谨,杜阳晒,王佐..miR-626下调HepG2细胞载脂蛋白(a)的表达[J].中国动脉硬化杂志,2017,25(7):661-665,5.基金项目
国家自然科学基金项目(81070221) （81070221）
