摘要
Abstract
Aim To investigate the effect and mechanism of Klotho on lipopolysaccharide (LPS) induced cardiomyocyte damage.Methods H9c2 cells were pretreated with different concentrations of Klotho protein,then treated with 1 g/L LPS for 6 hours,and cardiomyocyte damage was estimated by detecting the content of lactate dehydrogenase (LDH) and cell viability.Inflammation was estimated by detecting the content of tumor necrosis factor-α (TNF-α),interleukin-β(IL-β),IL-6.The status of oxidative stress was measured by malondialdehyde (MDA),superoxide dismutase (SOD),glutathione peroxidase (GSH-Px).Cell apoptosis was measured with flow cytometry.The expression of nuclear factor-κB (NF-κB)p65 and p-NF-κB p65 at protein levels were measured with Western blot assay.Results Klotho can alleviate LPS-induced decrease of cell viability,inhibit the release of LDH,TNF-α,IL-β,IL-6,down-regulate the content of MDA,enhance the activity of SOD,GSH-Px,suppress cell apoptosis and p-NF-κB p65 protein expression (P＜ 0.05).Conclusion Klotho can alleviate LPS-induced cardiomyocyte damage.And its mechanism may be related to the downregulation of NF-κB activation,and protect H9c2 cells from LPS-induced inflammation,oxidative stress and cell apoptosis.关键词
Klotho/心肌损伤/氧化应激/细胞凋亡/核因子κBKey words
Klotho/Cardiomyocyte damage/Oxidative stress/Apoptosis/NF-κB分类
医药卫生
