中国肺癌杂志2017，Vol.20Issue(7)：452-458,7.DOI:10.3779/j.issn.1009-3419.2017.07.03
MiR-218通过抑制Robo1的表达影响肺癌细胞迁移侵袭
MiR-218 Inhibits Migration and Invasion of Lung Cancer Cell by Regulating Robo1 Expression
陈平 1赵云龙 1李英杰1
作者信息
- 1. 100048 北京,解放军总医院第一附属医院
- 折叠
摘要
Abstract
Background and objective To explore the function and the potential molecular mechanism of miR-218 in lung cancer cell. Methods The expression of miR-218 mRNA was determined by real-time PCR in lung cancer tissues, ad-jacent tissues and lung cancer cells. Transwell assay was used to detect the migration and invasion of A549 cell after transfected with Anti-miR-218 or negative control and HC4006 cell after transfected with miR-218 mimics and miR-218 negative control. Targetscan and MiRanda were used to calculate the potential targets of miR-218 and Luciferase reporter assay was performed to identify that the Robo1 was one target genes of miR-218. Transwell assay was used to detect whether miR-218 regulated the invasion of lung cancer cell transfected with anti-miR-218 or negative control via Robo1. Results The expression of miR-218 in the lung cancer tissues was significantly lower than that in the adjacent tissues (P<0.05). Inhibition of miR-218 improved the migration and invasion of A549 cell. Overexpression of miR-218 suppressed the migration and invasion of HCC4006 cell. The co-transfection of anti-miR-218 or miR-218 mimics and the Robo13′UTR increased or reduced the luciferase activity of Robo1 compared with the control group (P<0.05). Inhibition of miR-218 and Robo1 recovered the invaded cells of A549. Overexpression of miR-218 and inhibition of Robo1 reduced the number of the invased cells of HCC4006. These results sug-gested that miR-218 banded Robo1 directly and inhibited lung cancer cell invasion by targeting Robo1. Conclusion MiR-218 inhibited the migration and invasion of lung cancer cells through regulating Robo1 expression.关键词
肺肿瘤/miR-218/Robo1/迁移/侵袭Key words
Lung neoplasms/miR-218/Robo1/Migration/Invasion引用本文复制引用
陈平,赵云龙,李英杰..MiR-218通过抑制Robo1的表达影响肺癌细胞迁移侵袭[J].中国肺癌杂志,2017,20(7):452-458,7.
