局解手术学杂志2017，Vol.26Issue(7)：474-478,5.DOI:10.11659/jjssx.04E017032
术中扩张对大隐静脉桥血管狭窄相关基因表达的影响
Effects of intraoperative expansion on expression of stenosis-related genes in saphenous vein graft
摘要
Abstract
Objective To investigate the effect of high pressure distention on the expression of stenosis-related genes of saphenous vein graft(SVG) during the coronary artery bypass grafting(CABG).Methods The biopsy specimens of saphenous vein collected from 10 patients who have undergone CABG,were divided into expansion group and no-expansion group.Real-time PCR and immunohistochemical staining were performed for examination of mRNA and protein expression of VE-cad,Egr-1,VCAN respectively.Student's t and Chi-square test were used to do statistic analysis.Results The results of RT-PCR showed that the mRNA transcription of Egr-1,VCAN in the expansion group were statistically significantly higher than those in no-expansion group(P<0.05).The mRNA transcription VE-cad in expansion group was statistically significantly lower than that in the no-expansion group(P<0.05).The immunohistochemical staining results showed that the expression of Egr-1 and VCAN in expansion group were significantly stronger than those in no-expansion group,while the expression of VE-cad was significantly lower than no-expansion group.Conclusion The intraoperative expansion of SVG can increase the expression of stenosis-related genes Egr-1 and Versican,and decrease the expression of stenosis-related gene VE-cad,which may be related with the SVG stenosis after CABG.关键词
冠状动脉旁路移植术/VE-cad/Egr-1/Versican/大隐静脉桥血管/高压扩张Key words
coronary artery bypass grafting/VE-cad/Egr-1/VCAN/saphenous vein graft/high pressure distention分类
医药卫生引用本文复制引用
庞磊,孟镔,李曦哲,施盛,叶一舟..术中扩张对大隐静脉桥血管狭窄相关基因表达的影响[J].局解手术学杂志,2017,26(7):474-478,5.基金项目
上海市科委生药处引导项目(114119b1600) （114119b1600）
