局解手术学杂志2017，Vol.26Issue(7)：479-482,4.DOI:10.11659/jjssx.03E017049
Luminex液态芯片技术在克罗恩病诊断与病情监测中的应用
Application of Luminex liquid chip technology in diagnosis and monitoring for patients with Crohn's disease
摘要
Abstract
Objective This study aimed to evaluate the clinical significance of detecting cytokine expression with Crohn's disease(CD) by Luminex liquid chip.Methods A total of 76 patients with CD and 50 healthy volunteers as healthy control group were recruited in this study.The expression of cytokines IL-2,IFN-γ,TNF-α,IL-4,IL-6,IL-10 and IL-17A were detected by Luminex liquid chip according to the instruction of MILLIPLEX MAP Kit.Enzyme-linked immunosorbent assay(ELISA) was used to detect the levels of anti-saccharomyces cerevisiae antibodies(ASCA).Then the relationship and differences among these groups were analyzed.Results Except IL-17A,the level of IL-2,IFN-γ,TNF-α,IL-4,IL-6,IL-10 was higher in the CD group than those in control group.The serum levels of IFN-γ,TNF-α and IL-6 in patients with active stage of CD group were significantly higher than those with remission stage and healthy control group.The level of TNF-α and IL-6 in moderate and severe stages were higher than those in slight stage.The serum level of ASCA had significant difference between CD group and control group.Compared with ASCA negative group,the ASCA positive patients have a higher level of IL-6 and TNF-α.ConclusionThe cytokines like IL-6 and TNF-α is correlated with CD severity,which is important for inflammatory activity and progression of CD.Altogether,these results demonstrated that the dynamic change of cytokines had a clear relativity in stage of CD,which is very imperative for diagnosis and clinical appraisal of CD.关键词
Luminex液态芯片技术/克罗恩病/抗酿酒酵母抗体/细胞因子Key words
Luminex liquid chip technology/Crohn's disease/anti-saccharomyces cerevisiae antibodies/cytokines分类
医药卫生引用本文复制引用
戴观荣,周单,丁洪亮,杨晓强..Luminex液态芯片技术在克罗恩病诊断与病情监测中的应用[J].局解手术学杂志,2017,26(7):479-482,4.基金项目
广东省科技计划项目(2014A020212735) （2014A020212735）
