局解手术学杂志2017，Vol.26Issue(7)：483-487,5.DOI:10.11659/jjssx.01E017061
Ad-shRNA-NgR转染EAE大鼠脑组织安全性评估
Safety evaluation of Ad-shRNA-NgR in brain tissue of EAE rats
张艳 1秦新月 2张敏 1贺曦 1张家玉3
作者信息
- 1. 重庆市急救医疗中心,重庆市第四人民医院神经内科,重庆 400010
- 2. 重庆医科大学附属第一医院神经内科,重庆 400016
- 3. 第三军医大学第二附属医院神经内科,重庆 400037
- 折叠
摘要
Abstract
Objective To observe the survival of EAE rats after recombinant adenovirus NgR specific RNA interference(Ad-shRNA-NgR) transfected the brain tissue of the experimental autoimmune encephalomyelitis(EAE),and provide the basis for EAE intervention.Methods EAE rats were randomly divided into high,medium,low and control groups(20 rats in each group).The lateral ventricle of EAE rats were injected with a titer of 1×1011 pfu/mL,1×1010 pfu/mL and 1×109 pfu/mL Ad-shRNA-NgR.The survival of EAE rats at third and seventh days after injection was observed.Results The survival rate of EAE rats of the high titer group was significantly lower than those of the middle titer group and low titer group at third and seventh days after Ad-shRNA-NgR transfected into EAE brain tissue.There was no significant difference in survival rate in middle titer group,low titer group and control group.Conclusion The titer of Ad-shRNA-NgR is safe in the experiment of EAE rats from 1×1010 pfu/mL to the range of 1×109 pfu/mL.关键词
腺病毒/实验性自身免疫性脑脊髓炎/转染/安全性/动物模型Key words
adenovirus/experimental autoimmune encephalomyelitis/transfection/safety/animal model分类
医药卫生引用本文复制引用
张艳,秦新月,张敏,贺曦,张家玉..Ad-shRNA-NgR转染EAE大鼠脑组织安全性评估[J].局解手术学杂志,2017,26(7):483-487,5.
