中国康复理论与实践2017，Vol.23Issue(7)：745-749,5.DOI:10.3969/j.issn.1006-9771.2017.07.001
电针预处理对脑缺血再灌注损伤大鼠神经功能和缺血半暗区Tolls样受体4、核转录因子κB蛋白的影响
Effects of Electroacupuncture Pretreatment on Neurological Function, and Expression of Toll-like Receptor 4 and Nuclear Factor κB in Ischemic Penumbra in Rats after Cerebral Ischemia-reperfusion Injury
摘要
Abstract
Objective To investigate the effect of electroacupuncture (EA) pretreatment on neurological function, and the expression of Toll-like receptor 4 (TLR4) and nuclear factorκB (NF-κB) protein in ischemic penumbra after cerebral ischemia-reperfusion (CIR) injury. Methods A total of 36 male Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12) and EA group (n=12). The later two groups were occluded their right middle cerebral arteries for two hours and reperfused. The EA group was pretreated with EA at Baihui (GV20) for two weeks before modeling. They were assessed with modified Neurological Severity Scores (mNSS), the injury in ischemic brain was detected with HE staining, and the expression of TLR4 and NF-κB protein in ischemic penumbra were detected with Western blotting, 24 hours after reperfusion. Results The score of mNSS decreased (P<0.05), the injury of brain tissue ameliorated, and the expression of TLR4 and NF-κB decreased in ischemic penumbra (P<0.05) in EA group compared with those in the model group. Conclu-sion EA pretreatment can reduce the injury and improve the neurological function in rats after CIR by down-regulating the expression of TLR4 and NF-κB protein in ischemic penumbra.关键词
脑缺血再灌注损伤/电针/预处理/神经功能/Tolls样受体4/核转录因子κB/大鼠Key words
cerebral ischemia-reperfusion injury/electroacupuncture/pretreatment/neurological function/Toll-like receptor 4/nuclear factorκB/rats分类
医药卫生引用本文复制引用
叶涛,朱路文,唐强,李宏玉,梁碧莹,张继瑶,郑婷婷..电针预处理对脑缺血再灌注损伤大鼠神经功能和缺血半暗区Tolls样受体4、核转录因子κB蛋白的影响[J].中国康复理论与实践,2017,23(7):745-749,5.基金项目
1.国家自然科学基金项目(No.81503666) （No.81503666）
2.黑龙江省自然科学基金项目(No.QC2015103) （No.QC2015103）
3.哈尔滨市科技创新人才研究专项基金项目(青年后备人)(No.2014RFQGJ150) （青年后备人）
4.黑龙江中医药大学领军人才计划项目(No.2012RCL02) （No.2012RCL02）
5.黑龙江中医药大学研究生创新科研项目(No. yjscx2016037). （No. yjscx2016037）
