中国康复理论与实践2017，Vol.23Issue(7)：750-755,6.DOI:10.3969/j.issn.1006-9771.2017.07.002
电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马CA1区突触超微结构的影响
Effects of Electroacupuncture at Baihui and Shenting on Learning-memory Function and Ultrastructure in Hip-pocampal CA1 in Cerebral Ischmeia-reperfusion Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture at Baihui (GV20) and Shenting (GV24) on learning-memory function and ultrastructure in hippocampal CA1 region of rats after cerebral ischmeia-reperfusion. Methods A total of 25 male Sprague-Dawley rats were randomly divided into sham group (n=6) and operation group (n=19). The operation group was occluded the left middle cerebral arter-ies with modified Longa's methods for 90 minutes and reperfused, and twelve qualified rats of them were randomly divided into model group (n=6) and electroacupuncture group (n=6), the later accepted electroacupuncture at Baihui and Shenting for seven days. They were as-sessed with Longa's scores, and tested with Barnes maze. Their cerebral infarct volume was tested with magnetic resonance imaging T2-weighted image. The ultrastructure of synapse in hippocampal CA1 region was observed with transmission electron microscope. Results Compared with the model group, the Longa's score improved (P<0.05), the infarct volume decreased (P<0.01), the average escape latency decreased (P<0.01) and the times entering the wrong hole decreased (P<0.001) in the electroacupuncture group. Under the transmission elec-tron microscope, the number of synapse decreased in the model group, with the structure damage and vesicles sparse;compared with the model group, the number of synapse increased in the electroacupuncture group, with clear and complete structure and rich vesicles. Conclu-sion Electroacupuncture at Baihui and Shenting can improve the learning-memory function in rats after cerebral ischmeia-reperfusion, which may relate to improvement of synaptic plasticity and ameliorating ultrastructure in hippocampal CA1 region.关键词
脑缺血再灌注/电针/突触/超微结构/学习记忆/海马/大鼠Key words
cerebral ischmeia-reperfusion/electroacupuncture/synapse/ultrastructure/learning-memory/hippocampus/rats分类
医药卫生引用本文复制引用
宋长明,黄佳,林冰冰,杨敏光,张秀峰,柳维林,陶静,陈立典..电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马CA1区突触超微结构的影响[J].中国康复理论与实践,2017,23(7):750-755,6.基金项目
1.国家自然科学基金项目(No.81403462) （No.81403462）
2.福建省教育厅省属高校项目(No.JK2014022). （No.JK2014022）
