中国临床药理学与治疗学2017，Vol.22Issue(6)：606-610,5.
映山红花总黄酮对大鼠神经元及EA.hy926内皮细胞超极化作用及机制
Effect and mechanism of total flavones of rhododendra on membrane hyperpolarization in rat hippocampal neurons and EA.hy926 cells
摘要
Abstract
AIM:To observe the effect and mechanism of total flavones of rhododendra (TFR) on membrane hyperpolarization in rat hippocampal neurons and EA.hy926 vascular endothelial cells.METHODS:Rat hippocampal neurons and EA.hy926 cells were cultured in vitro;the changes in fluorescence intensity of endothelial cells and rat neurons were measured by DiBAC4 (3) fluorescent dyes through the calcium ion imaging system with the excitation wavelength of 488 nm to reflect the membrane potential.RESULTS:Compared with control group,TFR (270 mg/L and 810 mg/L) could markedly reduce intensity of fluorescence in EA.hy926 cells,and the effect could be inhibited by BKCa specific inhibitor IbTX,the co-application of IKCa inhibitor ChTX and SKCa inhibitor apamin,while DL-propargylglycine (PPG) couldn't;Compared with Control group,TFR (270 mg/L and 810 mg/L) could obviously reduce the intensity of fluorescence in rat hippocampal neurons and this effect could also be blocked by IbTX.CONCLUSION:TFR can cause membrane hyperpolarization in EA.hy926 cells and rat hippocampal neurons;the possible mechanism is related to activating KCa channels.关键词
映山红花总黄酮/内皮细胞/海马神经元/钙激活钾离子通道/细胞膜电位Key words
total flavones of rhododendra/endothelial cells/hippocampal neurons/Ca2 +-sensitive K + channels/cell membrane potential分类
医药卫生引用本文复制引用
张洁,陈志武..映山红花总黄酮对大鼠神经元及EA.hy926内皮细胞超极化作用及机制[J].中国临床药理学与治疗学,2017,22(6):606-610,5.基金项目
国家自然科学基金项目(81374002,81173596) （81374002,81173596）
