中国临床药理学与治疗学2017，Vol.22Issue(6)：611-616,6.
白芍总苷对非酒精性脂肪性肝病大鼠HMGB1、RAGE通路的调控作用
Effect of total glucosides of paeony regulate HMGB1, RAGE pathway on nonalcoholic fatty liver disease in rats
摘要
Abstract
AIM:To observe the regulatory effect of total glucosides of paeony (TGP) on the HMGB1 and RAGE pathway (NAFLD) in rats with nonalcoholic fatty liver disease.METHODS:High fat and high fructose diet were administered to induce NAFLD rat models.The model rats were randomly divided into NAFLD group,TGP high (200 mg · kg-1 · d-1) and low dose group (100 mg · kg-1· d-1),Metformin group (200 mg · kg-1 · d-1),Silybin group (200 mg· kg-1 · d-1),and another blank control group was established.the rats total Cholesterol (TC),low density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C) and triglyceride (TG),free fatty acid (FFA),alanine aminotransferase (ALT),aspartate amino shifting enzyme (AST),fasting blood glucose (FBG),postprandial 2 hours blood glucose (2 hBG),insulin (fins) were observed 6 weeks after medication,and the insulin resistance index (HO-MA-IR) and liver index were thence calculated.Western blot was applied to detect the high mobility group protein 1 (HMGB1) and the receptor of advanced glycation end products (RAGE) protein in liver tissue.RESULTS:Compared with model group,the contents of 2 hBG,fins,HOMA-IR,LDL-C,TC,TG,FFA,ALT,AST,GST were decreased in high and low dose TGP groups (P ＜ 0.05 or P ＜ 0.01).High and low dose of TGP groups presented fair effect in antagonizing insulin resistance,lowering lipid and blood glucose as well as improving liver function.Also,TGP high and low dose group can down-regulate the expressions of HMGB1 (P ＜0.01) and RAGE (P ＜0.05) proteins.CONCLUSION:TGP can improve the metabolism of glucose and lipid,antagonize the insulin resistance,enhance the insulin sensitivity and improve the role of liver function in NAFLD rats through inhibition of HMGB1,RAGE signaling pathway.关键词
白芍总苷/非酒精性脂肪性肝病/信号通路/高迁移率族蛋白1/晚期糖基化终产物受体Key words
total glucosides of paeony/nonalcoholic fatty liver/signaling pathway/high mobility group box-1 protein/receptor for advanced glycation end-products protein分类
医药卫生引用本文复制引用
杨以琳,郑琳颖,古伟明,潘竞锵,武昕,黎耀俊..白芍总苷对非酒精性脂肪性肝病大鼠HMGB1、RAGE通路的调控作用[J].中国临床药理学与治疗学,2017,22(6):611-616,6.基金项目
2013年广东省科学技术厅科技计划项目(第二批-18) （第二批-18）
广州市卫生局中医药中西医结合科技项目(20142A011003) （20142A011003）
