中国临床药理学与治疗学2017，Vol.22Issue(6)：617-621,5.
子宫内膜异位症模型大鼠背根神经节神经元中TRPV1、TRPA1表达及其意义
Expression and significance of TRPV1 and TRPA1 in DRG neurons of rats with endometriosis
摘要
Abstract
AIM:To investigate the expression and significance of transient receptor potential channel protein V1 (TRPV1) and transient receptor potential channel protein A1 (TRPA1) in dorsal root ganglion (DRG) neurons in rats with endometriosis.METHODS:Female,mature unmated SpragueDawley healthy 40 rats were divided into model group and sham operation group.Model group underwent autologous transplantation methods to establish the rat model of endometriosis,while the sham operation group underwent open surgery.The expressions of DRG TRPV1 and TRPA1 were detected.And the hot plate pain threshold and tail flick latency change of rats were observed.RESULTS:The positive expression rates of TRPV1 and TRPA1 in the model group were significantly higher than those in the sham operation group (P ＜ 0.05).Before the operation,hot plate pain threshold and tail flick latency of two groups were similar (P ＞ 0.05);after the operation,the hot plate pain threshold and tail flick latency of rats in the model group decreased significantly (P ＜0.05),and were significantly less than those in the sham operation group (P ＜ 0.05).Before the TRPV1,TRPA1 antagonists treatment,the hot plate pain threshdd and tail flick latency were similar in the two groups (P ＞ 0.05),and after the antagonists treatment,the results were the same.In the model group,before and after the use of TRPV1,TRPA1 antagonist,hot plate pain threshold,tail flick latency had no significant change (P ＞ 0.05).CONCLUSION:TRPV1 and TRPA1 are highly expressed in the rat model of endometriosis,and the expression of TRPV1 and TRPA1 in the rat model of endometriosis can be decreased by using TRPV1 and TRPA1 antagonist,and the sensitivity to pain is decreased.关键词
子宫内膜异位症/动物模型/背根神经节神经元/瞬时受体电位通道蛋白V1/瞬时受体电位通道蛋白A1Key words
endometriosis/animal model/dorsal root ganglion neurons/transient receptor potential channel protein V1/transient receptor potential channel protein A1分类
医药卫生引用本文复制引用
刘建刚,王晓波..子宫内膜异位症模型大鼠背根神经节神经元中TRPV1、TRPA1表达及其意义[J].中国临床药理学与治疗学,2017,22(6):617-621,5.基金项目
浙江省自然科学青年基金(LQ13H040001) （LQ13H040001）
