中国临床药理学与治疗学2017，Vol.22Issue(6)：627-632,6.
小分子干扰RNA抑制HLX1表达对急性髓系白血病细胞侵袭的影响及机制探讨
Inhibitory effect of HLX1 on proliferation and invasion of acute myeloid leukemia cell
摘要
Abstract
AIM:To explore the effect of HLX1 on the proliferation and invasion ability in acute myeloid leukemia cell U937.METHODS:The HLX1 mRNA-targeting siRNA was transfected to U937 cells,the mRNA and protein expression of HLX1 were assessed by RT-PCR and Western blot,respectively.MTT assay and Transwell chamber assay were used to observe the proliferation and invasion ability of U937 cell after being transfected with HLX1 mRNA-targeting siRNA.RESULTS:Compared with the control group,HLX1 siRNA transfection reduced the level of HLX1 mRNA in U937 cells after 24 h,the inhibition rate was (70.0 ±2.7)％ (P ＜ 0.01);HLX1 siRNA transfeetion also reduced the expression of HLX1 protein in U937 cells after 48 h,the inhibition rate was (81.0 ± 3.1)％ (P ＜ 0.01);HLX1 transfection group cell proliferation was inhibited significantly (P ＜ 0.01);The invasion cells of HLX1 siRNA (22.0 ± 2.3) were significantly lower than that of control group (66.0 ± 5.0) (P ＜ 0.01),the expression of PAK1 protein was decreased in HLX1 transfection group (P ＜ 0.01).CONCLUSION:SiRNA can significantly inhibit the proliferation and invasion of acute myeloid leukemia cell by down-regulating the expression of HLX1.关键词
急性髓系白血病细胞/U937/同源异型盒基因/RNA干扰/侵袭Key words
acute myeloid leukemia cell/U937/HLX1/RNA interference/invasion分类
医药卫生引用本文复制引用
沈健,林颖超,朱夏吟..小分子干扰RNA抑制HLX1表达对急性髓系白血病细胞侵袭的影响及机制探讨[J].中国临床药理学与治疗学,2017,22(6):627-632,6.基金项目
浙江省医药卫生项目(2017195761) （2017195761）
