中国临床药理学与治疗学2017，Vol.22Issue(6)：633-639,7.
大黄素对实验性自身免疫性甲状腺炎小鼠辅助型T细胞亚群的影响
Effects of emodin on T helper cell subsets of experimental autoimmune thyroiditis mice
摘要
Abstract
AIM:To investigate the protective effect of emodin on thyroid in experimental autoimmune thyroiditis (EAT) mice,and its mechanism.METHODS:EAT animal model was induced by iodine on non-obese diabetic (NOD) mice.Four weeks after modeling,experimental groups were treated with emodin with different doses.The TgAb level in plasma and thyroid inflammation were detected eight weeks after modeling to evaluate the protective effect of emodin on EAT mice.T cell subset in peripheral blood and spleen were detected by flow cytometry.IL-4 and IFN-γwere measured by ELISA method.RESULTS:(1) The histopathological study revealed that inflammatory infiltration in thyroid was significant reduced compared with control group (P ＜ 0.01).(2) Levels of plasma TgAb were significant increased in each group after modeling,and the increasing amplitudes in emodin treated groups were significantly less than that in model group (P ＜ 0.01).(3) After modeling,levels of plasma IL-4,IFN-γ and the cell frequencies of CD3+ CD4+ IL-4 +,CD3+CD7+IFN-γ+,CD3+CD8+IL-4+and CD3+CD8+IFN-γ cells in peripheral blood monocyte and splenic lymphocyte were significant increased in each group compared with the control group (P ＜0.01).While the increasing amplitudes in emodin treated groups were less than that in model group.CONCLUSION:The EAT model is viable through an excessive iodine-induced method in NOD mice,and emodin shows a certain inhibitory effect on autoimmune response in EAT mice.This inhibitory effect could be performed by inhibiting the secretion of IFN-γ and IL-4 in CD4+ and CD8+ T cells,and thereby inhibiting the autoimmune response in EAT mice.关键词
实验性自身免疫性甲状腺炎/大黄素/Th细胞/NOD小鼠Key words
experimental autoimmune thyroiditis (EAT)/emodin/Th cell/NOD mice分类
医药卫生引用本文复制引用
吴可人,叶志鹏,金法,方蓉,徐涛,李宁..大黄素对实验性自身免疫性甲状腺炎小鼠辅助型T细胞亚群的影响[J].中国临床药理学与治疗学,2017,22(6):633-639,7.基金项目
浙江省中医药科技计划资助(2014ZA037) （2014ZA037）
