中国临床药理学与治疗学2017，Vol.22Issue(6)：650-653,4.
原花青素对AD大鼠学习记忆及CREB蛋白表达的影响
Effect of oligomeric proantho cyanidins on learning and memory and CREB expression of Alzheimer disease rats
周水妹 1瞿伟 2姚建标 2徐露3
作者信息
- 1. 杭州市萧山区中医骨伤科医院中药房,杭州311261,浙江
- 2. 浙江中药与天然药物研究院,杭州310052,浙江
- 3. 重庆医科大学药学院,重庆400016
- 折叠
摘要
Abstract
AIM:To explore the effect of oligomeric proantho cyanidins (OPC) on learning and memory and cAMP-response element binding protein (CREB) expression of Alzheimer disease (AD) rats.METHODS:Forty elder rats were randomly divided into four groups,i.e.the blank control group,model group,OPC high dose group and OPC low dose group.Except the normal group,rats of the other groups were cultivated into AD models by basal forebrain-injure.Morris water maze was applied to detect the learning and memory ability.Double anti-body sandwich method was used to test the activity of AchE and CHAT;Western blot was used to detect the expression of CREB protein.RESULTS:Compared with blank control group,the escape latency and distance of search were significantly longer;the search time and exploring ratio were significantly lower;the activity of AchE was significantly higher;the activity of ChAT and the expression of CREB were significantly lower.Compared with model group,OPC high dose group and OPC low dose group presented shorter escape latency and search distance,combined with longer exploring time and higher exploring ratio.The activity of AchE was significantly decreased while the expression of ChAT and CREB protein were significantly increased (P ＜ 0.05,P ＜ 0.01).CONCLUSION:OPC can improve the learning and memory of AD rats by decreasing AchE and increasing ChAT and CREB expression.关键词
阿尔茨海默病/原花青素/学习记忆/环腺苷酸应答原件结合蛋白Key words
Alzheimer disease/oligomeric proantho cyanidins/learning and memory/CREB分类
医药卫生引用本文复制引用
周水妹,瞿伟,姚建标,徐露..原花青素对AD大鼠学习记忆及CREB蛋白表达的影响[J].中国临床药理学与治疗学,2017,22(6):650-653,4.
