中国临床药理学与治疗学2017，Vol.22Issue(6)：654-658,5.
UTP23基因在上皮性卵巢癌紫杉醇耐药细胞中的表达及对紫杉醇化疗耐药的影响
Expression of UTP23 gene in epithelial ovarian cancer paclitaxel resistant cells and its effect on paclitaxel resistance to chemotherapy
童月红 1邵明君 1胡旻 1胡美旭 1张琳 1竺鹏飞2
作者信息
- 1. 金华市中心医院妇科,金华321000,浙江
- 2. 上虞市人民医院妇科,上虞312000,浙江
- 折叠
摘要
Abstract
AIM:To investigate the expression of UTP23 gene in epithelial ovarian cancer paclitaxel resistant cells and its effect on the resistance to paclitaxel chemotherapy.METHODS:The reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot were used to detect the expression level of UTP23,P-gp,MDR1 mRNA in epithelial ovarian cancer paclitaxel resistant cells;specific UTP23-siRNA was used to transfect epithelial ovarian cancer paclitaxel resistant cells;the expression of UTP23 gene after transfection was detected by RT-PCR and Western blot method;the effect of UTP23-iRNA on the growth of epithelial ovarian cancer paclitaxel resistant cells and drug resistance were detected by MTT method.RESULTS:The expression level of UTP23 gene mRNA in the resistant group was significantly lower than that in the sensitive group (P ＜ 0.05);the expression levels of P-gp mRNA and MDR1 mRNA in the resistant group were significantly higher than those in the sensitive group (P ＜ 0.05);compared with the non transfection group and the negative control group,the levels of UTP23 mRNA in the transfection group were significantly increased (P ＜ 0.05);compared with the negative control group,there was no significant difference of the level of UTP23 mRNA and protein level in the non transfection group (P ＞ 0.05);compared with the non transfection group and the negative control group,the expression levels of P-gp mRNA and MDR1 mRNA were significantly decreased in the transfection group (P ＜ 0.05);compared with the negative control group,there was no significant difference of the expression level of P-gp and MDR1 in mRNA in the transfection group (P ＞ 0.05);Paclitaxel on UTP23-siRNA after transfection,the concentration of IC50 in epithelial ovarian cancer resistant cell in the transfection group was significantly higher than those of the non transfection group and negative control group (P ＜ 0.05).CONCLUSION:The expression level of UTP23 gene in epithelial ovarian cancer paclitaxel resistant cells is low;it may be involved in the resistance of paclitaxel cells to paclitaxel in epithelial ovarian cancer by indirectly regulating the expression of MDR1 and P-gp.关键词
上皮性卵巢癌/UTP23基因/紫杉醇耐药细胞/耐药Key words
epithelial ovarian cancer/UTP23 gene/paclitaxel resistant cells/drug resistance分类
医药卫生引用本文复制引用
童月红,邵明君,胡旻,胡美旭,张琳,竺鹏飞..UTP23基因在上皮性卵巢癌紫杉醇耐药细胞中的表达及对紫杉醇化疗耐药的影响[J].中国临床药理学与治疗学,2017,22(6):654-658,5.
