中国临床药理学与治疗学2017，Vol.22Issue(6)：659-663,5.
MiR-148a在国人血浆中的分布及与高脂血症的关联性探讨
Relevance of plasma distribution of microRNA-148a in Chinese population and hyperlipidemia
摘要
Abstract
AIM:To detect the distribution of miR-148a level in Chinese people's plasma,and to explore the different expression level of miR-148a in plasma between healthy subjects and hyperlipidemia patients.METHODS:According to the clinical trial scheme,plasma samples of 52 patients with hyperlipidemia and 77 cases of healthy subjects were collected.RT-qPCR was used to determine the expression of miR-148a level.Shapiro-Wilk test as a way of normality test to the experimental data.Two independent sample t-test or one-way ANOVA was used to analyze the difference between groups.RESULTS:129 cases of subjects were quartile grouped by miR-148a level,and the △Ct of miR-148a were 1.65 ±0.80,3.91 ±0.64,5.68 ±0.61 and 8.21 ± 1.16,respectively.The difference between groups was statistically significant (P ＜ 0.05).Whereas,the △Ct of miR-148a of the healthy subjects group and patients with hyperlipidemia group were 4.81 ± 2.75 and 4.84 ± 2.26,respectively.There was no statistically significant difference between the two groups (P ＞ 0.05).CONCLUSION:The expression of miR-148a in Chinese people's plasma is normally distributed,and the difference between four groups is statistically significant.There is no difference between healthy subjects group and patients with hyperlipidemia group,suggesting that miR148a may not relate to hyperlipidemia.关键词
miR-148a/表达水平/分布/RT-qPCR/高脂血症Key words
miR-148a/expression level/distribution/Real-time quantitative polymerase chain reaction/hyperlipidemia分类
医药卫生引用本文复制引用
李西,靳高凤,刘志军,李新华,刘名义,张红,熊玉卿..MiR-148a在国人血浆中的分布及与高脂血症的关联性探讨[J].中国临床药理学与治疗学,2017,22(6):659-663,5.基金项目
国家自然科学基金项目(81260508) （81260508）
江西省“赣鄱英才555工程”人才资助计划(18000046) （18000046）
