中国临床药理学杂志2017，Vol.33Issue(11)：992-995,4.DOI:10.13699/j.cnki.1001-6821.2017.11.010
单唾液酸神经节苷脂注射液联合依达拉奉注射液对抗脑缺血性神经细胞损伤的作用
Effects of monosialoganglioside injection combined with edaravone injection against cerebral ischemic neuron injury in rats
摘要
Abstract
Objective To study the effects of monosialoganglioside combined with edaravone in the treatment of cerebral ischemic neuronal injury in rats.Methods Healthy SD rats were randomly divided into model group and experimental group (monosialin ganglioside 20 mg · kg-1 + edaravone 3 mg · kg-1),each group of 21 rats.The experimental group was injected one,twice a day respectively.The model group using the same amount of 1％ saline,the two groups were administered 14 days.Animal model of focal cerebral ischemia-reperfusion was made by middle cerebral artery occlusion.Using the image analysis system,according to the method of positioning the cerebral ischemic semi-dark bag in the same field of vision,the upper middle cerebral artery occlusion of the same side of the parietal lobe of the upper part of the cortex is defined as a semi-dark band.The silt was observed by immunity.Comparison of the average absorbance and positive area of 3-phosphoinositidedependent(PDK1),phosphatidylinositol-3-kinase (PI3 K),serine-threonine protein kinase (Akt) and glycogen synthase kinase-3β (GSK-3β) protein immunoreactive cells in histochemical method.Results The expression of the PI3K protein,Aktprotein,PDK1 protein were increased while expression of the GSK3β protein was decreased in the model group and the experimental group.After 7 days,positive unit area of PI3K protein,Aktprotein,PDK1 protein and GSK3β protein in the model group and the experimental group were (19.58 ± 1.13),(26.91 ± 0.90) mm2;(15.98 ± 0.48),(22.87 ± 1.20)mm2;(17.97 ± 0.58),(26.02 ± 0.54) mm2;(22.03 ± 0.92),(14.02 ± 0.45) mm2,the difference between two groups was significant (all P ＜ 0.05).The average absorbance density of the four protein in the model group and the experimental group were 0.19 ±0.01,0.28 ±0.02;0.16 ±0.01,0.25 ±0.01;0.16 ±0.01,0.25 ±0.01;0.22 ±0.01,0.14 ±0.01,the difference between two groups was statistically significant (all P ＜0.05).Conclusion Single sialic acid ganglioside combined with edaravone against cerebral ischemia injury of nerve cells in the region.关键词
依达拉奉注射液/单唾液酸神经节苷脂注射液/脑缺血性神经损伤/丝氨酸-苏氨酸蛋白激酶Key words
edaravone injection/monosialoganglioside injection/cerebral ischemic injury/serine-threonine protein kinase分类
医药卫生引用本文复制引用
何士科,林莉,蔡学礼..单唾液酸神经节苷脂注射液联合依达拉奉注射液对抗脑缺血性神经细胞损伤的作用[J].中国临床药理学杂志,2017,33(11):992-995,4.基金项目
浙江省医药卫生研究计划基金资助项目(2014KYB314) （2014KYB314）
