中国临床药理学杂志2017，Vol.33Issue(11)：996-999,4.DOI:10.13699/j.cnki.1001-6821.2017.11.011
氧化苦参碱对胰岛素抵抗小鼠肝脂质转运酶的作用
Effect of oxymatrine on hepatic lipid transport in insulin-resistant of mice
摘要
Abstract
Objective To investigate the effect of oxymatrine on hepatic lipid transport in insulin resistant of mice.Methods C57BL/6J mice were selected as control group.Apolipoprotein E gene knockout (ApoE-/-)mice were fed with high-fat diet for 16 weeks to establish the model of insulin resistance,which were randomly divided into model group,low,middle and high dose experimental groups.The experimental groups were given a gavage of 25,50,100 mg · kg-1 oxymatrine,respectively.All groups were continuously administrated for 8 weeks.Hyperinsulinemic-euglycemic clamp test were measured.The expression levels of lipid transport enzyme regulated genes in the hepatic were examined by real-time fluorescence quantitative PCR and Western-blot.Results Compared with the control group,the contents of triglyceride (TG),cholesterol (TC) and fatty acid (FFA) in the liver of model group significantly increased.Compared with the control group,serum fasting blood glucose (FBG),TC,TG and FFA levels significantly increased.Fasting insulin (FINS) were lower.Glucose infusion rate (GIR) (16.46 ± 1.62) mU · kg-1 · min-1 in model group was significantly lower than that control group.Lipoprotein lipase (LPL) and fatty acid translocase (FAT/CD36) mRNA expression in model group increased,which were 2.21 ±0.35 and 3.61 ±0.42.Carnitine palmitoyl transferase 1 (CPT1) and peroxisome proliferator-activated receptor α (PPARα) mRNA expression in model group decreased,which were 0.28 ±0.04 and 0.23 ±0.04.The protein expressions of LPL,FAT/CD36 (0.93 ±0.15,0.72 ±0.08) in model group increased,while CPT1,PPARα (0.31 ± 0.05,0.22 ± 0.04) in model group decreased.The differences in above factors were statistically significant (all P ＜ 0.05).After administration,the contents of TG,TC and FFA,blood lipid,insulin levels decreased in the liver of experimental group.The GIR values of middle and high dose experimental groups were 20.13 ± 1.84 and 21.33 ±2.26 respectively.LPL,FAT/CD36 expression were down -regulation,while CPT1,PPARa expression were up-regulation.The LPL mRNA expressions of middle and high dose experimental groups were 1.60 ±0.21 and 1.19 ±0.14 respectively,and the FAT/CD36 mRNA expressions were 1.63±0.19 and 1.78±0.24.CPT1 were0.87 ±0.12,0.90±0.15,and PPARα were0.68±0.11,0.55 ±0.08 respectively.The LPL protein expressions were 0.61 ± 0.08,0.45 ± 0.06,FAT/CD36 were 0.33 ± 0.04,0.36 ±0.05,CPT1 were 0.96 ±0.13,0.99 ±0.17 and PPARα were 0.65 ±0.10,0.71 ±0.11 respectively in the two groups.The differences of above indexes were statistically significant (all P ＜ 0.05).Conclusion Oxymatrine can adjust the lipid transport enzyme in hepatic,thus improving insulin resistance in mice.关键词
氧化苦参碱/高脂饲养/胰岛素抵抗/脂质转运酶Key words
oxymatrine/high fat diet/insulin resistance/lipid transport enzyme分类
医药卫生引用本文复制引用
王超,张会欣,邢邯英,王杏,张哲..氧化苦参碱对胰岛素抵抗小鼠肝脂质转运酶的作用[J].中国临床药理学杂志,2017,33(11):996-999,4.基金项目
国家重点基础研究发展计划(973计划)基金资助项目(2012CB518606) （973计划）
河北省重大医学科研基金资助项目(zd2013002 （）
zd2013004) （）
