中国临床药理学杂志2017，Vol.33Issue(12)：1094-1097,4.DOI:10.13699/j.cnki.1001-6821.2017.12.007
肝硬化患者外周血甲胎蛋白与丙酮酸激酶M2和白蛋白mRNA的表达及临床意义
Expression of peripheral α-fetoprotein, pyruvate kinase isoenzyme type M2 and albumin detection on liver cirrhosis and its clinical value
摘要
Abstract
Objective To explore the expression of peripheral mRNA expression level of α-fetoprotein (AFP),pyruvate kinase isoenzyme type M2(PKM2),albumin(Alb) in patients with liver cirrhosis and its clinical value.Methods A total of 89 cases of liver cirrhosis patients were selected as hepatocirrhosis groups,60 healthy people were selected as the control group.The 89 cases of liver cirrhosis group accorded to serum HBV-DNA detection results were divided into viral replication group (53 cases) and non viral replication group (36 cases).The 5 mL of peripheral blood was extracted.Plasma mRNA expression level of AFP,PKM2,Alb were detected by real-time quantitative PCR in hepatocirrhosis group and control group.Serum HBV-DNA levels were detected by fluorescence quantitative PCR in hepatocirrhosis group.The correlation between plasma AFP,PKM2,Alb mRNA and HBV-DNA load was analyzed.And the corresponding ROC curves were drawn accorded to the relative expression of AFP,PKM2 and Alb mRNA.Results The AFP mRNA relative expression in hepatocirrhosis group was 13.6 ±2.1,PKM2 mRNA was 6.4 ±0.5,Alb mRNA was 15.1 ±2.5;while that in control group were 7.6 ± 0.7,1.4 ± 0.2,7.2 ± 0.5 respectively.Compared with control group,it was significantly higher than that in control group (P ＜ 0.05).AFP mRNA relative expression in viral replication group was 15.3 ± 4.2,PKM2 mRNA was 7.6 ± 0.7,Alb mRNA was 18.2 ± 4.5,and non viral replication group was 11.1 ± 2.4,4.6 ± 0.3,10.5 ± 1.7,respectively.The AFP,PKM2,ALB mRNA expression in viral replication group was significantly higher than that in non viral replication group (P ＜ 0.05).Correlation analysis showed that AFP mRNA was positively correlated with HBV-DNA (r =0.401,P ＜ 0.05),PKM2 mRNA was positively correlated with HBV-DNA (r =0.380,P ＜0.05),and Alb mRNA was positively correlated with HBV-DNA (r =0.607,P ＜0.001).To AFP,PKM2,ALB mRNA expression to draw the ROC curve,the results showed that AFP mRNA in ROC curve area of 0.885,the optimal critical value was 8.7,corresponding to the sensitivity of 84.9％,specificity was 84.3％;PKM2 mRNA ROC curve area of 0.879,the optimal critical value was 3.4,corresponding to the sensitivity was 84.1％,the specificity was 81.7％;area under the Alb mRNA ROC curve was 0.921,the optimal critical value was 9.5,the corresponding sensitivity was 88.1％,the specificity was 96.8％.Conclusion AFP,PKM2,Alb mRNA in liver cirrhosis patients' plasma were significantly increased,and it can provide reference for liver cirrhosis diagnosis by detecting AFP,PKM2,Alb mRNA in peripheral blood.关键词
甲胎蛋白/丙酮酸激酶M2/白蛋白/肝硬化Key words
α-fetoprotein/pyruvate kinase isoenzyme type M2/albumin/liver cirrhosis分类
医药卫生引用本文复制引用
童杨雯,张青..肝硬化患者外周血甲胎蛋白与丙酮酸激酶M2和白蛋白mRNA的表达及临床意义[J].中国临床药理学杂志,2017,33(12):1094-1097,4.基金项目
国家自然科学基金资助项目(30570070) （30570070）
