中国临床药理学杂志2017，Vol.33Issue(12)：1102-1105,4.DOI:10.13699/j.cnki.1001-6821.2017.12.009
酶联免疫吸附法测定肿瘤性贫血患者的血清细胞因子水平及其发病机制研究
Determination the cytokineslevels of plasma by ELISA in patients with cancer relate anemiaand study onits nosogenesis mechanisms
摘要
Abstract
Objective To explore the the mechanism of tumor necrosis factor-α (TNF-α),etythropoietin (EPO) and interferon-γ(IFN-γ)on cancer relate anemia(CRA).Methods A total of 97 cases of malignant tumor patients were divided into treatment group (tumor with anemia,76 cases) and control group (tumor without anemia,21 cases);76 cases of CRA paitents accorded to the parameters of iron metabolism were divided into treatment Ⅰ group (29 cases) and treatment Ⅱ group (47 cases),another 50 healthy volunteers were selected as the normal group.The serum erythropoietin (EPO),ferritin (SF) level were detected by radioimmunoassay method.The serum TNF-α,IFN-γ level were detected by enzyme-linked immune adsorption method.Extracted of normal bone marrow,conventional cultured,added different concentrations of TNF-α,IFN-γ,red blood cell colony forming units (CFU-E) in bone marrow were counted,added different concentration of recombinant human erythropoietin (rhEPO) in culture system,the concentration of CFU-E were counted.Results The serum levels of TNF-α in treatment Ⅰ group,treatment Ⅱ group and control group were (137.8 ± 13.2),(241.4 ± 19.8),(113.7 ± 12.6) ng· L-1;levels of EPO in that three groups were(513.7 ±37.1),(268.8 ±21.9),(94.6 ± 11.4) ng· L-1;levels of IFN-γ in that three groups were (191.5 ± 16.5),(289.7 ± 20.3),(157.9 ± 14.7)ng ± L-1.The three factors were significantly higher than that of normal group (51.3 ± 8.4),(56.8 ± 7.1),(57.4 ± 7.8) ng · L-1 with significantly (P ＜ 0.05).The serum EPO in treatment Ⅱ group was significantly higher than that in control group,but was significantly lower than that in treatment Ⅰ group with significantly (P ＜ 0.05).The serum levels of TNF-α and IFN-γ in the treatment Ⅱ group were significantly higher than those in the treatment Ⅰ group and control group with significantly (P ＜ 0.05).In vitro cell culture results showed that TNF-e,IFN-γ with inhibition effect on normal bone marrow CFU-E,and this inhi-bitory effect with concentration dependent-manner with the increased of concentration.The inhibitory effect of CFU-E cells in bone marrow was stronger.By added rhEPO in the culture system,the inhibition effect of TNF-α,IFN-γ could be improved in 2 × 10-10 ～32 × 10-10 mol · mL-1 range,the higher the concentration of rhEPO,the more obvious the improvement effect on the inhibition of TNF-α,IFN-γ;but still unable to return to normal level.Conclusion The regulatory factor of TNF-α,INF-γ effect on EPO,cause the reaction of bone marrow erythroid to EPO relative reduction,maybe one of the mechanisms of cancer relate anemia.关键词
肿瘤性贫血/肿瘤坏死因子-α/红细胞生成素/干扰素-γ/铁代谢Key words
cancer relate anemia/tumor necrosis factor-α/etythropoietin/interferon-γ/iron metabolism分类
医药卫生引用本文复制引用
杨斌,张建武..酶联免疫吸附法测定肿瘤性贫血患者的血清细胞因子水平及其发病机制研究[J].中国临床药理学杂志,2017,33(12):1102-1105,4.基金项目
国家自然科学基金资助项目(81172043) （81172043）
