中国临床药理学杂志2017，Vol.33Issue(12)：1143-1148,6.DOI:10.13699/j.cnki.1001-6821.2017.12.020
甘草水煎液和内生菌止咳祛痰作用的比较研究
Comparative study of antitussive and expectorant effects between Glycyrrhiza decoction and endophytes
摘要
Abstract
Objective To compare antitussive and expectorant effects between endophytes from Glycyrrhiza uralensis and decoction,and filtrate effective strains.Methods A total of 84 Kunming mice were randomly divided into blank group,positive control group,Glycyrrhiza decoction group,3 groups of endophytes (JTYB006 group,JTYB029 group,JTYF023 group).There are two batches of mice altogether.Blank group was given the injection of 0.9％ NaCl.Positive control group was given 30 mg · kg-1 codeine phosphate tablets.Glycyrrhiza decoction group was given 1.3 g · kg-1 wild Glycyrrhiza decoction.Three groups of endophytes were respectively given 1.3 g · kg-1 powder of endophytes.Six groups were given the corresponding dose by intragastric administration once a day,for 7 days.1 hour after the last medication,the mice cough models were respectively induced by ammonia and SO2.The antitussive effects were evaluated by median effective dose time (EDT50),R-value,the incubation period of cough,the times of cough within 2 minutes,inhibition ratio of cough and extension ratio of cough.70 Wistar rats were randomly divided into 7 groups,these were blank group,model group,positive control group,Glycyrrhiza decoction group,3 groups of endophytes(JTYB006,JTYB029,JTYF023).The rat model of phlegm blocking in lung was established by inhaleing SO2 and clod wind for 10 days.10 days later,the blank group was given the injection of 0.9％ NaCl,positive control group was given 0.08 mg · kg-1 compound bulbus fritilillariae cirr hosate and ammonium chloride tablets,Glycyrrhiza decoction group was given 0.95 g · kg-1 wild Glycyrrhiza decoction,3 groups of endophytes were respectively given 0.95 g · kg-1 powder of endophytes.Seven groups were given the corresponding dose by intragastric administration once a day for 7 days.The general activities,the amount of phlegm and pathological of lung tissues were measured.In addition,the levels of nitric oxide (NO),tumor necrosis factor (TNF-α),interleukin-17 (IL-17) and IL-23 were measured by enzyme-linked immunosorbent (ELISA) method.Results The EDT50 in blank group was 13.00 s,which in positive control,Glycyrrhiza decoction,JTYB006,JTYB029,JTYF023 groups were 30.09,20.32,18.45,25.03,16.52 s,the EDT50 of all drug intervention groups extended.Positive control,Glycyrrhiza decoction and JTYB029 groups had the obvious effect (R ＞ 150),JTYB006 had the effect of relieving cough (130 ＜ R ＜ 150),JTYF023 was invalidity(R ＜ 130).For cough caused by SO2,the incubation period of cough in blank group was (14.67 ± 7.20) s and positive control,Glycyrrhiza decoction,JTYB006,JTYB029 groups (28.00 ± 8.15),(25.83 ±9.35),(20.58 ±6.52),(26.50 ±5.32)s with statistically significant difference (P ＜0.01 or P ＜0.05).The times of cough within 2 minutes in blank group was(78.92 ± 19.06) and positive control,Glycyrrhiza decoction,JTYB006,JTYB029 groups were (47.42 ± 19.96),(57.67 ± 19.04),(63.15 ± 15.30),(44.25 ± 16.87) with statistically significant difference (P ＜ 0.01 or P ＜ 0.05).Compared with Glycyrrhiza decoction,JTYB006 and JTYF023 had remarkable short on the incubation period of cough,JTYB029 had remarkable reduction on the times of cough within 2 minutes(P ＜0.01 or P ＜0.05).In model,positive control,Glycyrrhiza decoction and JTYB029 groups,the amount of phlegm were (14.47 ± 3.30),(7.26 ± 4.55),(8.45 ± 3.78),(8.23 ± 1.19) mm,the levels of NO were (51.57±8.33),(30.02 ±2.65),(39.10 ±9.63),(39.25 ± 10.20)μmol · L-1,the levels of TNF-α were (654.80±56.40),(282.56 ±70.81),(400.24 ±78.03),(410.86 ±68.74) ng· mL-1,the levels of IL-17 were (66.28 ±9.34),(39.10 ±6.27),(50.20 ±9.05),(48.70 ± 10.28)pg · mL-1,the levels of IL-23 were (29.04 ±3.55),(16.02 ±3.60),(20.11 ±2.96),(21.25 ±3.40)pg · mL-1 Compared with the model group,Glycyrrhiza decoction and JTYB029 had remarkable decreasions in the terms of the amount of phlegm and the levels of NO,TNF-o,IL-17 and IL-23 (P ＜ 0.01 or P ＜ 0.05).Conclusion By filtrating,2 endophytes from Glycyrrhiza uralensis (JTYB006,JTYB029) have antitussive effects,and 1 endophytes from Glycyrrhiza uralensis (JTYB029) has expectorant effects.The antitussive and expectorant effects have no significant difference between wild Glycyrrhiza'decoction and JTYB029.关键词
甘草/内生菌/止咳/祛痰Key words
Glycyrrhizae Radix Et Rhizoma/endophytes/antitussive/expectorant分类
医药卫生引用本文复制引用
杨志军,邓毅,曼琼,王勇,杨秀娟,赵妮,刘靓..甘草水煎液和内生菌止咳祛痰作用的比较研究[J].中国临床药理学杂志,2017,33(12):1143-1148,6.基金项目
国家自然科学基金资助项目(81360633) （81360633）
