中国美容医学2017，Vol.26Issue(7)：32-36,5.
TGR5多种配体抑制高脂饮食小鼠肥胖及高血糖的效果对比研究
Comparison of the Effects of TGR5 Ligands on Obesity and Hyperglycemia in High Fat Diet Mice
摘要
Abstract
Objective To screen out the best ligand by comparing the weight loss and hypoglycemic effects of various ligands for bile acid receptor TGR5 in diet-induced obese mice. Methods Wild-type mice of 6 weeks old were fed with HF or the control regular chow diet for 10 weeks. Then mice fed with HF were randomly divided into seven groups. One group were kept feeding with HF. The other groups were switched to HF+OA(H)、HF+OA(L)、HF+CDCA、HF+CA、HF+DCA、HA+LCA for additional 10 weeks. Glucose tolerance test and serum insulin levels were detected after 20 weeks. Hematoxylin and eosin staining was performed for standard histological examination in liver and adipose tissues. Expression of genes related to glucose metabolism in adipose tissues was measured by Real-time PCR. Mice body weights were monitored during the entire process. Results TGR5 activation by its ligands decreased the body weight, enhanced insulin sensitivity in mice and up-regulated the expression of genes related to fatty acid synthesis and oxidation in adipose tissues, with OA(H), CDCA and CA affected significantly. Conclusion OA(H)、CDCA and CA were identified as being effective at reducing weight, improving glucose tolerance, reducing serum insulin levels, relieving liver lipid deposition and regulating the expression of fat metabolism related genes.关键词
TGR5配体/褐色脂肪/肥胖/糖脂代谢Key words
TGR5 ligands/BAT/obesity/glycolipid metabolism分类
医药卫生引用本文复制引用
陈小松,闫柳,陈瑛,李向禹,郭志辉,李铭..TGR5多种配体抑制高脂饮食小鼠肥胖及高血糖的效果对比研究[J].中国美容医学,2017,26(7):32-36,5.基金项目
国家自然科学基金(No.81372092) （No.81372092）
国家自然科学基金(No.81671930) （No.81671930）
福建省科技厅高校产学研合作项目(No.2016Y4003) （No.2016Y4003）
福建省发改委产业技术联合创新项目(No. 02011601) （No. 02011601）
福建省财政专项项目(No.010110002) （No.010110002）
