中国免疫学杂志2017，Vol.33Issue(7)：967-970,4.DOI:10.3969/j.issn.1000-484X.2017.07.002
Rab7基因沉默对巨噬细胞中R848激活的细胞因子及MAPK信号通路影响
Effects of Rab7 gene silencing on cytokine and MAPK signal pathway activated by R848 in macrophage
摘要
Abstract
Objective:To investigate the effect of Rab7 on cytokine induced by TLR7 (Toll like receptor-7) R848 activated in Raw264.7,and discusses the influence of Rab7 on MAPK signal transduction.Methods: TLR7 downstream cytokines such as TNF-α,IL-6,IFN-α,IFN-β and IP-10 activated by R848 were detected through Q-PCR in Rab7 silenced mouse macrophages,and then analysis of phosphorylation of MAPK determined with Western blot showed the effect of Rab7 on signal transduction of MAPK.Results: Rab7 inhibit production of cytokine activated by TLR7,and also,Rab7 had an inhibitory effect on MAPK signal pathway.Conclusion: The experimental results further illustrate that the Rab7 is the TLR7 signal transduction pathway negative regulatory factor,and to participate in MAPK signaling pathway.关键词
Rab7/TLR7/Raw264.7细胞/MAPKKey words
Rab7/TLR7/Raw264.7 cell/MAPK分类
医药卫生引用本文复制引用
邹凯,云小乐,康鸿斌,王雪,张晓慧,谢基明,王玉珍..Rab7基因沉默对巨噬细胞中R848激活的细胞因子及MAPK信号通路影响[J].中国免疫学杂志,2017,33(7):967-970,4.基金项目
本文受国家自然科学基金(31270922,81360394)和内蒙古自治区硕士研究生创新基金(520151012907,520151012908,2015ms08 124)资助. （31270922,81360394）
