中国免疫学杂志2017，Vol.33Issue(7)：1035-1038,4.DOI:10.3969/j.issn.1000-484X.2017.07.016
资木瓜中莽草酸对大鼠腹腔肥大细胞脱颗粒及抗炎作用研究
Degranulation and anti-inflammatory effects of shikimic acid in Chaenomeles speciosa on peritoneal mast cells in rats
摘要
Abstract
Objective:To investigate the effect of Chaenomeles speciosa total extract shikimic acid(SA) on the degranulation of compound 48/80(C48/80) stimulated rat peritoneal mast cells and the anti-inflammatory.Methods: Qualitative analysis of shikimic acid in the total extract of Chaenomeles speciosa by high performance liquid phase;RPMC were identified by toluidine blue,immunohistochemistry and electron microscopy;CCK-8 method was used to detect the proliferation of RPMC in each concentration group.The release rate of β-hexosidase was determined by substrate method.The content of histamine in supernatant was detected by ELISA.Results: SA had no significant effect on the growth of RPMC at the concentration of 0-90 μg/ml.Compared with the positive control group,SA could effectively inhibit the release of β-hexosidase and inhibit the secretion of histamine.Conclusion: SA inhibits the release of histamine in inflammatory mediators by inhibiting the degranulation of RPMC stimulated by C48/80,and then exerts anti-inflammatory effects.关键词
资木瓜/莽草酸/RPMC/β-己糖苷酶/组胺Key words
Chaenomeles speciosa/Shikimic acid/RPMC/β-hexos1 minidase/Histamine分类
医药卫生引用本文复制引用
郑倩倩,董甜甜,李世刚,柳蔚,喻玲玲,陈先勇,王金龄..资木瓜中莽草酸对大鼠腹腔肥大细胞脱颗粒及抗炎作用研究[J].中国免疫学杂志,2017,33(7):1035-1038,4.基金项目
本文为三峡大学医学院国家自然科学基金资助项目(No.81673665,81274116). （No.81673665,81274116）
