中国免疫学杂志2017，Vol.33Issue(7)：1043-1047,5.DOI:10.3969/j.issn.1000-484X.2017.07.018
未成熟树突状细胞快速分离与体外诱导培养及其鉴定研究
Isolation,induced culture and identification of immature dendritic cells in vitro
摘要
Abstract
Objective:To establish a fast method for the generation of immature dendritic cells(DCs) from human peripheral blood mononuclear cells(hPBMCs) in vitro.Methods: High purity human CD14+ monocytes were collected using density Ficoll gradient centrifugation and MACS beads sorting system.iDCs(Immature DC) were induced after cultured with rhGM-CSF and rhIL-4 on the fourth day.Fluorescence activated cell sorting(FACS) was used to identify cell surface markers(CCR5) and capabilities of antigen uptake of iDCs on the fourth day.Ordinary optical microscope,scanning electron microscopy and transmission electron microscopy were used to observe surface and internal structure of iDCs on the fourth day of culture conditions.Results: FACS result shows that the purity of CD14+ monocytes collected from hPBMCs were more than 94%.The antigen uptake capability and CD195 of iDCs was detected on the fourth day of cultured conditions.Typical surface and internal structure characteristics of iDCs were observed.Conclusion: Rapid induction culture is an effective method for obtaining a large number of iDC with typical characteristics in vitro,and can be used for further experimental study.关键词
单核细胞/未成熟树突状细胞/诱导培养/鉴定Key words
Monocytes/Immature dendritic cell/Induced culture/Appraisal分类
医药卫生引用本文复制引用
朱娜,王海桃,李昌,李海僖,郭焱,金宁一..未成熟树突状细胞快速分离与体外诱导培养及其鉴定研究[J].中国免疫学杂志,2017,33(7):1043-1047,5.基金项目
本文为国家自然科学基金(31472197)、病原微生物生物安全国家重点实验室开放课题(SKLPBS1435)、北京市自然科学基金(5152023)和吉林省青年科研基金项目(20140520173JH)资助项目. （31472197）
