中国农业大学学报2017，Vol.22Issue(7)：24-33,10.DOI:10.11841/j.issn.1007-4333.2017.07.03
十字花科植物PIN3基因调控元件及表达分析
Regulating elements and expression analysis of PIN3 gene in Brassicaceae family
摘要
Abstract
A total of 18 homologous PIN3 genes were identified in 17 species in Brassicaceae family based on genomic data available including Arabidopsis thaliana,Capsella rubella,Brassica rapa and Brassica oleraceaetc.Gene sequences were subjected to bioinformatics analysis and light-,hormone-response elements and stress-response elements are differently contained in the gene promoter as cis elements.The auxin-response and geotropism response elements are conservative among the 18 homologous genes.The promoter of PIN3 (CbPIN3) from Capsella bursapastoriswas recombined with GUS reporter system CbPIN3pro ∷ GUS and transformed into Arabidopsis thaliana to investigate the specific expression model of the homologous gene.By comparison of GUS staining,it was found that the CbPIN3 gene displayed same expression pattern with the chip data of PIN3 in Arabidopsis.However,the expression pattern of CbPIN3 varies in some tissue.This might be related to the differentiation of auxin polar transport regulation in plant morphogenesis.The differences in expression pattern of PIN3 between Capsella bursa-pastoris and Arabidopsis thaliana were adaptive evolution in the Brassicaceae family.关键词
十字花科/PIN3基因/启动子元件/基因表达Key words
Brassicaceae family/PIN3 gene/promoter elements/expression分类
农业科技引用本文复制引用
李玉军,赵燕,黄丽华,汤宾,黄妤,张学文..十字花科植物PIN3基因调控元件及表达分析[J].中国农业大学学报,2017,22(7):24-33,10.基金项目
湖南省教育厅高校重点实验室开放项目(15K060) （15K060）
