中国农业大学学报2017，Vol.22Issue(7)：61-70,10.DOI:10.11841/j.issn.1007-4333.2017.07.08
骆驼斯氏副柔线虫雌虫和雄虫比较转录组学分析
Comparative transcriptome analysis of female and male adults of Parabronema skrjabini in camel
摘要
Abstract
Aiming to find sex-determining genes,transcriptome profile of female and male adults of Parabronema skrjabini in camel were investigated.Samples of female and male were analyzed using Illumina HiSeq2000TM RNA sequencing platform and their transcriptome libraries were constructed.After quality filtering and de novo assembling,functional annotation and bioinformatics analysis were carried out.The results showed that:A total of 6 430 differentially expressed genes between female and male were obtained,of which 2 131 and 4 299 of genes were up-and downregulated,respectively.And 341 328 and 714 differentially expressed genes were annotated into biological process,cellular component and molecular function and 41 functional categories.KEGG analysis showed that 1 116 differentially expressed genes were assigned to 198 KEGG pathways,and clustered significantly in immuno regulation,oocyte maturation and so on.Functional cluster analysis indicted that reproductive development,oviposition and reproductive behavior were high enriched in female adult stage,whilst the sperm main protein,serine/threonine specific protein phosphorylas etc.were high enriched in male adults stage.Moreover,we identified ten the significant sex-determining genes,such as Tab-3,Tab-5 and Fem-1,eight gender-specific expression genes of male adult and four gender-specific expression genes of female adult.These genes were necessary to decide nematode gender.In this study,the biological characteristics of differentially expressed genes were predicted by bioinformatics,which involved in functional genes and metabolic pathways.This study provides a foundation for the further whole genome sequence analysis,drug target prediction and vaccine candidate gene screening.关键词
斯氏副柔线虫/转录组/差异表达基因/生物信息学分析Key words
Parabronemas krjabini/transcriptome/differentially expressed genes/bioinformatics analysis分类
农业科技引用本文复制引用
冯陈晨,王文龙,红梅,刘宁,呼和巴特尔..骆驼斯氏副柔线虫雌虫和雄虫比较转录组学分析[J].中国农业大学学报,2017,22(7):61-70,10.基金项目
国家自然科学基金资助项目(31260603) （31260603）
