中国农业大学学报2017，Vol.22Issue(8)：133-144,12.DOI:10.11841/j.issn.1007-4333.2017.08.17
Vb生物膜反应器处理酵母废水的发酵特性研究
Study on fermentation characteristics of yeast wastewater treated by biofilm reactor
摘要
Abstract
To explore the capability of anaerobic biofilm reactor to treat high concentration organic waste water and the variation of microbial diversity in the reactor,biofilm reactor with carbon fiber carrier was used to handle yeast waste water by gradually raising feeding concentration.Hydraulic retention time (HRT) was controlled to 6 d in this study.The results showed that:The volumetric efficiency of gas production increased from 0.06 to 0.14 and 0.37 L/(L · d);During the process of upgrading,the influent COD concentration was 2 798 mg/L at startup phase and increased to 5 596 and 13 990 mg/L;The average methane contents were 57％,71％ and 74％ respectively,and the average COD removal rate were 45％,64％ and 65％ respectively.The reactor displayed strong resistance capability to organic loading shocks.When influent COD concentration continued increasing to 22 980 mg/L and the corresponding COD volume loading was 4.70 g/(L · d),the COD removal rate reduced to 49 ％,and pH was remained at 7.7,the average methane content was 66％ and the volumetric efficiency of gas production further increased to 0.58 L/(L · d).It was found that over feeding volume loading improved the volumetric efficiency of gas production,but also caused the decreasing of gas conversion efficiency.High-throughput sequencing results showed that marsh-microbes in the bioreactor gradually stabilized with the organic loading increasing and the operation time elapsing.And the main archaea and bacteria of microbial community in the reactor were Methanospirillum,Methanosaeta,Methanobacterium and Acidovorax,Sporosarcina,respectively.The proportion of dominant microorganisms was 58％.The proportions of archaea and bacteria of marsh liquid in start and stable phase were 69.23％,30.77％ and 66.86％,33.14％,respectively.The proportion of methanogens in archaea communities was raised from 33.11％ (initial stage) to 76.70％ (stable stage),and hydrogenotrophic methanogens became the dominant microorganism in archaea community which was increased from 24.67％ (initial stage) to 60.13％ (stable stage).关键词
酵母废水/沼气发酵/生物膜反应器/微生物群落结构/生物甲烷Key words
yeast wastewater/biogas fermentation/biofilm reactor/microbial community structure/bio-methane分类
农业科技引用本文复制引用
姚义,吕育财,李宁,郭金玲,田毅红,龚大春..Vb生物膜反应器处理酵母废水的发酵特性研究[J].中国农业大学学报,2017,22(8):133-144,12.基金项目
国家自然科学基金项目(31370506) （31370506）
湖北省自然科学基金项目(2014CFB666) （2014CFB666）
国家自然科学基金青年基金项目(31500422) （31500422）
三峡大学研究生科研创新基金项目(2015CX141) （2015CX141）
