中国农业科技导报2017，Vol.19Issue(7)：36-43,8.DOI:10.13304/j.nykjdb.2017.0019
基于SSR和ITS标记的杨树多态性及聚类分析
Populus Polymorphism and Cluster Analysis based on SSR and ITS Markers
摘要
Abstract
In order to study the poplar diversity and differences and connection among different molecular marker technology,this paper analyzed DNA polmorphism of 12 poplar samples by SSR and ITS markers,and conducted correlation analysis of these 2 markers on the basis of genetic distance.SSR marker indicated that 54 fragments were amplified by 13 pairs primers and polymorphic bands were 51.The PIC (polymorphism information content) value of these markers was varied from O.36 ～ 0.76,and could preferably distinguish different samples.The results of ITS analysis showed that the differences between tested poplar not only expressed in basic group,but also in sequence length.The results of all samples clustering analysis by these 2 marker methods showed that the two were consistent in faction classification.But there were differences between SSR and ITS markers in subtle classification,SSR marker could more accurately identify the plants with same source or similar individual plants than ITS marker.The correlation analysis of these 2 methods indicated that they were significantly correlated,the correlation coefficient R reached 0.593 (P=0.01,n=66,rα=0.314 966).Thus,SSR and ITS markers could all be used for poplar variety identification.Combining with the genetic differences of these 2 molecular markers,this paper provided technical basis for the rapid and accurate identification of poplars germplasm resources.关键词
杨树/SSR/ITS/聚类分析Key words
Populus/SSR/ITS/cluster analysis分类
农业科技引用本文复制引用
韩志校,张军,左力辉,董研,王进茂..基于SSR和ITS标记的杨树多态性及聚类分析[J].中国农业科技导报,2017,19(7):36-43,8.基金项目
国家林业局科技发展中心项目(XPC-2016004)资助. （XPC-2016004）
