中国农业科技导报2017，Vol.19Issue(7)：44-49,6.DOI:10.13304/j.nykjdb.2016.677
山东地区樱桃褐斑病病原鉴定及rDNA-ITS序列分析
Identification and ITS Sequence Analysis of Pathogen of Cherry Brown Leaf Spot in Shandong
摘要
Abstract
In order to clarify the pathogenic species of cherry brown leaf spot,this study isolated and purified 16 fungal strains by single spore separation method.It also collected typical morbidity leaf blades from Taian,Weifang and Yantai 3 regions.Identification of cherry brown leaf spot pathogen was carried out by pathogen morphology combined with molecular biology method.The ITS sequence analysis of this pathogen showed that it was aligned with Genbank and showed 97％ identity with Passalora arctostaphyli.The results of phylogenetic analysis showed that the pathogen was located at the same branch of phylogenetic tree with Passalora sp.and different from Pseudocercospora sp.According to its morphological characteristics and the sequence of internal transcribed spacer,the pathogen was identified as Passalora circumscissa,and ITS gene sequence was presented to GenBank database(Genbank Accession No.:KT428056).关键词
樱桃/褐斑病/ITS序列分析/鉴定Key words
cherry/brown leaf spot/ITS sequence analysis/identification分类
农业科技引用本文复制引用
孙杨,韩伟,孙玉刚,魏国琴,付全娟,杨兴华,宫庆涛..山东地区樱桃褐斑病病原鉴定及rDNA-ITS序列分析[J].中国农业科技导报,2017,19(7):44-49,6.基金项目
山东省自然科学基金培养基金项目(ZR2014CP017)资助. （ZR2014CP017）
