中国水产科学2017，Vol.24Issue(4)：690-697,8.DOI:10.3724/SP.J.1118.2017.16238
干露胁迫对长牡蛎基因组DNA甲基化的影响
Effects of air exposure on genomic DNA methylation in the Pacific oyster (Crassostrea gigas)
摘要
Abstract
The Pacific oyster (Crassostrea gigas) inhabits the intertidal zone and shows tolerance to air exposure conditions. Most marine invertebrates have been demonstrated to suffer large-scale mortality following sudden changes in osmolality, causing huge economic losses to commercial aquaculture. To explore the effect of air ex-posure on the genomic DNA methylation of marine shellfish, methylation polymorphisms in genomic DNA in the Pacific oyster were analyzed using the fluorescence-labeled methylation sensitive amplified polymorphism (F-MSAP) technique. The oysters were exposed to air individually for 0 d, 0.5 d, 1 d, 3 d, 5 d, 7 d, 9 d, and 11 d. The incidence of DNA methylation in adductor and gill tissue was 29.76% and 29.82%, respectively, in the control group (day 0). Compared with the control group, the level of total methylation in various groups under air expo-sure increased initially, after which it decreased. The total level of methylation on days 0.5, 1, 3, 5, 7, 9, and 11 was 36.59%, 38.86%, 43.02%, 39.30%, 51.13%, 46.79%, and 35.06%, respectively, in adductor tissues and 39.3%, 42.13%, 39.36%, 43.54%, 56.19%, 38.57%, and 28.99%, respectively, in gill tissues. The methylation level on day 7 was higher than that following other air exposure durations (P<0.05); at the end of the experiment (11 d), the methylation level nearly returned to that of the control. Moreover, data regarding genomic DNA methylation mu-tation patterns at CCGG sites were not consistent with cytosine methylation patterns and patterns of variation be-tween adductor and gill tissue; hypermethylation sites were more numerous than demethylation sites (P<0.05), indicating that remarkable changes occurred in the hypermethylation sites of the Pacific oyster. The results of the present study implied that the alteration of methylation patterns in C. gigas in response to air exposure might be strongly linked to stress resistance. These studies are fundamental to direct further research regarding DNA me-thylation in the aquatic shellfish epigenome, specific gene expression, and stress tolerance.关键词
长牡蛎/干露胁迫/DNA甲基化/荧光标记甲基化敏感扩增多态性(F-MSAP)Key words
Crassostrea gigas/air exposure/DNA methylation/fluorescence-labeled methylation sensitive ampli-fied polymorphism (F-MSAP)分类
农业科技引用本文复制引用
张鑫,李琪,于红,孔令锋..干露胁迫对长牡蛎基因组DNA甲基化的影响[J].中国水产科学,2017,24(4):690-697,8.基金项目
农业科技成果转化资金项目(2014GB2B020029) （2014GB2B020029）
泰山学者种业计划专家项目 （）
山东省科技发展计划项目(2014GHY115002) （2014GHY115002）
