中国水产科学2017，Vol.24Issue(4)：698-709,12.DOI:10.3724/SP.J.1118.2017.16243
墨西哥湾扇贝与华贵栉孔扇贝的远缘杂交
Reciprocal crosses between Argopecten irradians concentricus and Chlamys nobilis
摘要
Abstract
In this study, two scallops, Argopecten irradians concentricus and Chlamys nobilis, were used to inves-tigate the possibility of intergeneric distant hybridization in scallops. Using A. irradians concentricus as the fe-male parent and C. nobilis as male parent, we established four experimental groups—an orthogonal group MH, a reciprocal group HM, an A. irradians concentricus inbred group MM, and a C. nobilis inbred group HH—to in-vestigate the fertilization, early development, and growth and survival of reciprocal crosses between the two scal-lops. The results show that reciprocal crosses of A. irradians concentricus and C. nobilis can derive hybrids. For reciprocal cross groups MH and HM, the time required for the embryos to develop into D-shaped larva was less than that required for embryos in the inbred groups MM and HH (MH 18 h, HM 19 h, MM 21 h, and HH 22 h). The average fertilization rate and average hatching rate of the MH and HM groups were lower than those of the MM and HH groups. Under different temperature, salinity, and sperm concentration, the fertilization rate and hatching rate of reciprocal cross groups MH and HM were lower than those of the MM and HH groups. A com-parison of the growth of planktonic larvae among four experimental groups from day 2 to day 11 revealed that the shell length and height of the MH and HM groups were significantly higher than those of the MM and HH groups, with both showing obvious heterosis (P<0.01). Experiments designed to predict the larvae of the orthogonal and reciprocal groups on the basis of the inbred group in shell length and height revealed that the growth tendency of hybrid scallop can be predicted using the BP neural network model method, and the error rate of the predicted values was less than 4%.关键词
墨西哥湾扇贝/华贵栉孔扇贝/远缘杂交/胚胎发育/幼虫生长/BP神经网络模型/预测比较Key words
Argopecten irradians concentricus/Chlamys nobilis/reciprocal crosses/embryo development/larval growth/BP neural network/comparison of prediction分类
生物科学引用本文复制引用
潘奕达,封杰,刘行,王文杰,潘英..墨西哥湾扇贝与华贵栉孔扇贝的远缘杂交[J].中国水产科学,2017,24(4):698-709,12.基金项目
广西科学研究与技术开发计划项目(桂科攻14121006-2-5). （桂科攻14121006-2-5）
