中国水产科学2017，Vol.24Issue(4)：746-756,11.DOI:10.3724/SP.J.1118.2017.16307
解淀粉芽孢杆菌和胶红酵母复合菌对虹鳟生长性能及胃黏膜、肠黏膜菌群结构的影响
Effects of Bacillus amyloliquefaciens V4 and Rhodotorula mucilaginosa on growth and microbiota of gastric mucosa and intestinal mucosa in rainbow trout (Oncorhynchus mykiss)
摘要
Abstract
Probiotic dietary supplementation has been widely used and has positive effects on the growth perfor-mance and host health of different fish species. Little attention is paid to its influence on fish gastrointestinal mu-cosal microbiota. A feeding trial was conducted to investigate the potential effects of probiotic compounds of Ba-cillus amyloliquefaciens V4 and Rhodotorula mucilaginosa on the growth performance and gastric and intestinal mucosal microbiota of rainbow trout. A total of 360 rainbow trout with average body weight of (205.1±4.82) g were distributed in four equal groups in three replicates, and received one of the following experimental diets for 42 days: basal diet, basal diet supplemented with 5×106/5×107 CFU/g (T1, see tables 1 and 2), 1.5×107/1.5×108 CFU/g (T3, see tables 1 and 2), and 2.5×107/2.5×108 CFU/g (T5, see tables 1 and 2) of probiotic compounds. Significant im-provement in weight gain ratio, feed conversion ratio, and the specific growth rate (SGR) was found in T1 diet supplemented with probiotic compounds. Significantly decreased mortality was found in T1 and T3 diets. Poly-merase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was applied to compare the gastroin-testinal mucosal microbiota of different treatments. In gastric mucosa, 35.7±17.0 (C0), 37.0±3.5 (T1), 36.7±13.6 (T3), and 26.0±13.2 (T5) bands were found, with no significant differences (F=0.500, P=0.692). In intestinal mucosa, 23.3±5.8 (C0), 22.3±3.2 (T1), 16.7±8.0 (T3), and 24.7±7.4 (T5) bands were found, also with no significant diff-erences (F=0.916, P=0.475). The bacterial diversity of gastric mucosa increased with increasing probiotic concen-tration, but it decreased in T5. However, the bacterial diversity of intestinal mucosa decreased with increasing probiotic concentration, which showed recovery in T5. Using both the unweighted pair-group method with arith-metic means (UPGMA) clustering and principal component analysis (PCA) ordination, we found that the microbi-al community structures of gastric mucosa and intestinal mucosa were different. No significant influences were found on bacterial diversity and microbial community structure of intestinal mucosa of rainbow trout with addition of probiotic compounds of Bacillus amyloliquefaciens V4 and Rhodotorula mucilaginosa. The potential risks of high concentrations of probiotics should be paid more attention.关键词
解淀粉芽孢杆菌/胶红酵母/虹鳟/消化道黏膜菌群/PCR-DGGEKey words
Bacillus amyloliquefaciens/Rhodotorula mucilaginosa/Oncorhynchus mykiss/gastric and intestinal mu-cosal microbiota/PCR-DGGE分类
农业科技引用本文复制引用
王纯,孙国祥,刘志培,刘淑兰,王顺奎,刘鹰..解淀粉芽孢杆菌和胶红酵母复合菌对虹鳟生长性能及胃黏膜、肠黏膜菌群结构的影响[J].中国水产科学,2017,24(4):746-756,11.基金项目
国家自然科学基金资助项目(31472312 （）
41306152 （）
31402283) （）
现代农业产业技术体系建设专项资金项目(CARS-48) （CARS-48）
国家科技支撑计划课题(2014BAD08B09). （2014BAD08B09）
