中国输血杂志2017，Vol.30Issue(5)：454-458,5.DOI:10.13303/j.cjbt.issn.1004-549x.2017.05.004
血浆miR-122在抗-HCV阳性献血者中表达的动态分析
Dynamic monitoring of expression of miR-122 in anti-HCV positive blood donors
摘要
Abstract
Objective To dynamically monitor the expression of plasma miR-122 in anti-HCV positive blood donors and analyze the relationship between miR-122 levels and HCV diagnostic test results.Methods 133 anti-HCV reactive donors,24 healthy blood donors,15 anti-HCV reactive and HCV RNA positive followed up blood donors were included in the study.The level of miR-122 in plasma were measured using real-time quantitative RT-PCR,and the relationship between miR-122 levels and HCV RNA,COI were statistically analyzed.Results From discriminating anti-HCV+/HCV RNA+ positive blood donors from those anti-HCV +/HCV RNA-negative people,the AUC-ROC (the area under the ROC curve) was 0.830 (95％CI:0.755-0.889),and the sensitivity and specificity were 74.7％ and 82.4％,respectively.The expression of miR-122 in plasma was significantly decreased in 15 anti-HCV+/HCV RNA+ patients (P＜0.05).Conclusion MiR-122 is closely related to HCV infection,and decreased with time in blood donors of HCV RNA infection.关键词
丙型肝炎病毒/抗-HCV/血浆miR-122/HCV RNA/临界值指数/生物标志物Key words
Hepatitis C virus/Plasma miR-122/HCV RNA/COI/biomarker分类
医药卫生引用本文复制引用
王立林,杨宝成,朱为刚,卢亮,洪文旭,孙元璋,龙洁萍,曾劲峰,李雪梅..血浆miR-122在抗-HCV阳性献血者中表达的动态分析[J].中国输血杂志,2017,30(5):454-458,5.基金项目
深圳市科技创新委员会项目(JCYJ20140403093211510),深圳市科卫生计生系统科研项目(201401074,201401077) （JCYJ20140403093211510）
