中国输血杂志2017，Vol.30Issue(5)：525-528,4.DOI:10.13303/j.cjbt.issn.1004-549x.2017.05.026
核酸检测HBV DNA阳性献血者的追踪检测结果分析
Analysis of the results on tracking assay of the blood donors with positive HBV DNA in nucleic acid test
摘要
Abstract
Objective To provide a basis for the safety of clinical blood transtusion through termly tracking assay of the blood donors whose nucleic acid test (NAT) are positive,but negative in enzyme-linked immunosorbent assay (ELISA).Methods After the collections of blood donors’ specimen,they were assayed using two kinds of ELISA reagent.The eligible ones were assayed by 8 specimens in one group using Shanghai Grandsoo's nucleic acid test systemn.The reactive ones in the mixed test would be divided to be further tested,and the divided result would be the final report result,then termly tracking assay would be prepared for those blood donors whose NAT were positive.Results Among the 40 496 specimens from the blood donors,40 189 were eligible in ELISA,and 307 were ineligible.From the 40 189 specimens eligible in ELISA,22 were found to be HBV DNA positive.There was no specimen that were HCV RNA and HIV-1 RNA positive.Tracking jobs were accomplished for 17 blood donors whose HBV DNA were postrive,among which 14 were occult hepatitis B virus infections.There was no “window period” infection from blood donors.Conclusion There is still risk of blood transfusion after double ELISA.The safety level of clinical blood transfusion can be raised through NAT implementation.The tracking survey shows that OBI is predominant in HBV DNA positive blood donors.This is the main cause for the residual risk found in blood transfusion.关键词
核酸检测/献血者/追踪/隐匿性感染/HBVKey words
nucleic acid test/blood donors/tracking/occult infection/HBV分类
临床医学引用本文复制引用
来祝檩,韩惠云,张国平..核酸检测HBV DNA阳性献血者的追踪检测结果分析[J].中国输血杂志,2017,30(5):525-528,4.
