中国比较医学杂志2017，Vol.27Issue(7)：1-5,5.DOI:10.3969.j.issn.1671-7856.2017.07.001
Fkbp51基因敲除小鼠心脏与肝脏RNA表达谱系的分析比较
Analysis and comparison of RNA expression profiles in the heart and liver of Fkbp51 knockout mice
摘要
Abstract
Objective To study the function of Fkbp51 in the heart and liver by analyzing the differential RNA expression profiles in the wild-type mice (WT) and Fkbp51 knockout (KO) mice, and to elucidate the role of Fkbp51 gene in metabolic pathways in the heart and liver.Methods Using the second generation of high-throughput gene sequencing technology, the mRNA expression profiles of heart and liver were sequenced in WT and Fkbp51 KO mice.The data of sequencing of heart tissues were analyzed by DEGseq, and the results of sequencing of liver tissues were analyzed by BRB-Array Tools.The differential genes of the heart and liver in the mice were screened respectively.Gene ontology (GO) analysis and KEGG pathway analysis were performed to analyze the differentially expressed genes using the online tool DAVID.In addition, the differential genes of the two organ tissues were analyzed by Venn diagram.The interaction network of proteins was analyzed using the STRING database.Results (1) The absence of Fkbp51 led to changes in mRNA expressions of heart-related signal pathways such as vascular smooth muscle contraction, chemokine, retinol, and MAPK signaling pathways.(2) The lack of Fkbp51 mostly induced changes in cholesterol synthesis and metabolism, lipid metabolism, redox and other related genes and pathways in the liver.(3) In the heart and liver, Fkbp51 deletionresult ed in four co-differential genes, among them, down-regulation of Rnaset2b, Hmga1 and Fkbp51, while Cyp2b10 was down-regulated in the heart but up-regulated in the liver.All these proteins may interact with HSP90 protein and participat in the metabolism of heart and liver tissues.Conclusions Fkbp51 is involved in different metabolic and gene expression regulation pathways of heart and liver, and the roles are both independent and interrelated.关键词
Fkbp51基因敲除小鼠/心脏/肝脏/代谢/基因表达/信号通路Key words
Fkbp51 KO mouse/Liver/Heart/Metabolism/Gene expression/Signaling pathways分类
医药卫生引用本文复制引用
武光东,邱彬,王婷婷,刘云波,雍伟东..Fkbp51基因敲除小鼠心脏与肝脏RNA表达谱系的分析比较[J].中国比较医学杂志,2017,27(7):1-5,5.基金项目
北京市自然科学基金资助项目(NO.7164278) （NO.7164278）
国家科技重大专项(NO.2014ZX10004002-003-001) （NO.2014ZX10004002-003-001）
国家自然科学基金(NO.81272273) （NO.81272273）
中央级公益性科研院所基本科研业务费(NO.DWS201508,DWS201607). （NO.DWS201508,DWS201607）
